| Category | Antibody |
| Concentration | 2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form | Antigen affinity purified |
| Conjugation | Unconjugated |
| Clone | Polyclonal antibody |
| Recognised antigen | 1 / SPI1, PU |
| Host animal | Rabbit (Oryctolagus cuniculus) |
| Clonality | Polyclonal (rabbit origin) |
| Species reactivity | Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications | IHC-P |
| Recommended dilutions | 5-1ug/ml, IHC (Paraffin): 0 |
| Notes | 1 antibody should be determined by the researcher, Optimal dilution of the PU |
| Intented use | 1 antibodyis to be used only for research purposes and not for diagnostics, This PU |
| Uniprot # | P17947 |
| Purity | Antigen affinity |
| Description | Multiple transcript variants encoding different isoforms have been found for this gene, The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, The protein can also regulate alternative splicing of target genes, This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development, and regulates their expression in coordination with other transcription factors and cofactors, 1 is a protein that in humans is encoded by the SPI1 gene, Transcription factor PU |
| Immunogen | 1 antibody, 1/SPI1 were used as the immunogen for the PU, Amino acids 18-196 of human PU |
| Storage | Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the PU, 1 antibody may be kept for up to one month refrigerated at +4 degrees C, After reconstitution, For long-term |
| Localization | Nuclear |
| Properties | C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target | 1 / SPI1, PU |
| Short name | 1 Antibody / SPI1, PU |
| Technique | antibodies against human proteins, antibodies for, Antibody |
| Alternative name | 1 (Antibody to) / spleen focus forming virus (SFFV) proviral integration oncogene spi1, PU |
| Alternative technique | antibodies |
| Alternative to gene target | BT, DNA-templated and biological process this GO :0006357 and regulation of transcription from RNA polymerase II promoter and biological process this GO :0006366 and transcription from RNA polymerase II promoter and biological process this GO :0030098 and lymphocyte differentiation and biological process this GO :0030218 and erythrocyte differentiation and biological process this GO :0030225 and macrophage differentiation and biological process this GO :0030851 and granulocyte differentiation and biological process this GO :0035019 and somatic stem cell maintenance and biological process this GO :0043011 and myeloid dendritic cell differentiation and biological process this GO :0043565 and sequence-specific DNA binding and molecular function this GO :0043966 and histone H3 acetylation and biological process this GO :0044027 and hypermethylation of CpG island and biological process this GO :0045087 and innate immune response and biological process this GO :0045347 and negative regulation of MHC class II biosynthetic process and biological process this GO :0045646 and regulation of erythrocyte differentiation and biological process this GO :0045814 and negative regulation of gene expression, DNA-templated and biological process this GO :0045893 and positive regulation of transcription, DNA-templated and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0051525 and NFAT protein binding and molecular function this GO :0060033 and anatomical structure regression and biological process this GO :0090241 and negative regulation of histone H4 acetylation and biological process this GO :1902262 and apoptotic process involved in patterning of blood vessels and biological process, NFAT protein binding, OF and PU, SPI1 and IDBG-43260 and ENSG00000066336 and 6688, Sfpi1 and IDBG-190185 and ENSMUSG00000002111 and 20375, epigenetic and biological process this GO :0045892 and negative regulation of transcription, nuclei, this GO :0000122 and negative regulation of transcription from RNA polymerase II promoter and biological process this GO :0000790 and nuclear chromatin and cellular component this GO :0000978 and RNA polymerase II core promoter proximal region sequence-specific DNA binding and molecular function this GO :0000979 and RNA polymerase II core promoter sequence-specific DNA binding and molecular function this GO :0000980 and RNA polymerase II distal enhancer sequence-specific DNA binding and molecular function this GO :0000981 and sequence-specific DNA binding RNA polymerase II transcription factor activity and molecular function this GO :0001047 and core promoter binding and molecular function this GO :0001077 and RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription and molecular function this GO :0001078 and RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription and molecular function this GO :0001085 and RNA polymerase II transcription factor binding and molecular function this GO :0001205 and RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription and molecular function this GO :0001944 and vasculature development and biological process this GO :0002320 and lymphoid progenitor cell differentiation and biological process this GO :0002573 and myeloid leukocyte differentiation and biological process this GO :0003677 and DNA binding and molecular function this GO :0003700 and sequence-specific DNA binding transcription factor activity and molecular function this GO :0003705 and RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity and molecular function this GO :0003723 and RNA binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0006355 and regulation of transcription, this GO :0000978: RNA polymerase II core promoter proximal region sequence-specific DNA binding, this GO :0000978: RNA polymerase II core promoter proximal region sequence-specific DNA binding and also this GO :0000979: RNA polymerase II core promoter sequence-specific DNA binding and also this GO :0000980: RNA polymerase II distal enhancer sequence-specific DNA binding and also this GO :0000981: sequence-specific DNA binding RNA polymerase II transcription factor activity and also this GO :0001047: core promoter binding and also this GO :0001077: RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription and also this GO :0001078: RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription and also this GO :0001085: RNA polymerase II transcription factor binding and also this GO :0001205: RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription and also this GO :0003677: DNA binding and also this GO :0003700: sequence-specific DNA binding transcription factor activity and also this GO :0003705: RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity and also this GO :0003723: RNA binding and also this GO :0005515: protein binding and also this GO :0043565: sequence-specific DNA binding and also this GO :0051525: NFAT protein binding, this GO :0000979: RNA polymerase II core promoter sequence-specific DNA binding, this GO :0000980: RNA polymerase II distal enhancer sequence-specific DNA binding, this GO :0000981: sequence-specific DNA binding RNA polymerase II transcription factor activity, this GO :0001047: core promoter binding, this GO :0001077: RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription, this GO :0001078: RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription, this GO :0001085: RNA polymerase II transcription factor binding, this GO :0001205: RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription, this GO :0003677: DNA binding, this GO :0003700: sequence-specific DNA binding transcription factor activity, this GO :0003705: RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity, this GO :0003723: RNA binding, this GO :0005515: protein binding, this GO :0043565: sequence-specific DNA binding, this GO :0051525: NFAT protein binding, 1 and SFPI1 and SPI-1 and SPI-A, 58934 and IDBG-637435 and ENSBTAG00000021709 and 507100, spleen focus forming virus (SFFV) proviral integration oncogene spi1 |
| Identity | 11241 |
| Gene | SPI1 |
| Long gene name | Spi-1 proto-oncogene |
| Synonyms gene name | spleen focus forming virus (SFFV) proviral integration oncogene |
| Synonyms | PU, 1 SPI-A OF SFPI1 SPI-1 |
| Synonyms name | hematopoietic transcription factor PU, 1 31 kDa transforming protein |
| Locus | 11p11, 2 |
| Discovery year | 1988-07-04 |
| GenBank acession | X52056 |
| Entrez gene record | 6688 |
| Pubmed identfication | 1693183 |
| RefSeq identity | NM_003120 |
| Classification | ETS transcription factor family |
| Havana BLAST/BLAT | OTTHUMG00000150150 |
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