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RAB5 Antibody, FITC Conjugated

RAB5 Antibody, FITC Conjugated size: 0.1ml 333

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 333
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name FITC Conjugated, RAB5 Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenRAB5
SpecificityThis is a highly specific antibody against RAB5
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Subcellular locationCytoplasm
SourceKLH conjugated synthetic peptide derived from human RAB5
Gene ID number5868
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background CXC, Due to the presence of truncated sequences in this CD, GTPase activating proteins (GAPs) interact with GTP-bound Rab and accelerate the hydrolysis of GTP to GDP, Guanine nucleotide exchange factors (GEFs) interact with GDP-bound Rabs to promote the formation of the GTP-bound state, In mammals, In yeast, Lipid binding is essential for membrane attachment, Most Rab GTPases contain a lipid modification site at the C-terminus, Rab5 GTPases localize to early endosomes and regulate fusion of clathrin-coated vesicles to early endosomes and fusion between early endosomes, Rabs are further regulated by guanine nucleotide dissociation inhibitors (GDIs), The members of this subfamily are involved in endocytosis and endocytic-sorting pathways, This subfamily includes Rab5 and Rab22 of mammals, Ypt51/Ypt52/Ypt53 of yeast, Ypt51p family members similarly regulate membrane trafficking through prevacuolar compartments, a key feature of most Rab proteins, and RabF of plants, or CCX, the lipid modification site is not available for annotation, which facilitate Rab recycling by masking C-terminal lipid binding and promoting cytosolic localization, with sequence motifs CC, Rab5-related subfamily
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetRAB5 Conjugated
Short name FITC Conjugated, RAB5 Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, RAB5 (Antibody to)
Alternative techniqueantibodies
Identity 9783
Gene RAB5A
Long gene name RAB5A, member RAS oncogene family
Synonyms gene RAB5
Synonyms name RAS-associated protein RAB5A
Locus 3p24, 3
Discovery year 1990-01-22
Entrez gene record 5868
Pubmed identfication 1999336
RefSeq identity NM_004162
Classification RAB, member RAS oncogene GTPases
Havana BLAST/BLAT OTTHUMG00000129889

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