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RAG2 Antibody, ALEXA FLUOR 647

RAG2 Antibody, ALEXA FLUOR 647 size: 100ul 332

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES
Price 332
Size 100ul
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name ALEXA FLUOR 647 Conjugated, RAG2 Polyclonal Antibody
Also known asAnti-RAG2 PAb ALEXA FLUOR 647
CategoryConjugated Primary Antibodies
Conjugated with 647, ALEXA FLUOR®
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenRAG2
SpecificityThis is a highly specific antibody against RAG2
ModificationUnmodified
Modification SiteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceThis antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human RAG2
Gene ID Number5897
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Mouse, Rat, Human
Cross-reactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Background of the antigen 5'-phosphorylated ends, DNA cleavage occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, Histone H3 monoubiquitination is required for the joining step of V(D)J recombination, In addition to its endonuclease activity, In the RAG complex, RAG1 also acts as a E3 ubiquitin-protein ligase that mediates monoubiquitination of histone H3, RAG1 mediates the DNA-binding to the conserved recombination signal sequences (RSS) and catalyzes the DNA cleavage activities by introducing a double-strand break between the RSS and the adjacent coding segment, RAG2 is not a catalytic component but is required for all known catalytic activities, The RAG complex also plays a role in pre-B cell allelic exclusion, The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps, The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T lymphocytes through rearrangement of different V (variable), a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B lymphocytes, and J (joining) gene segments, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, in some cases D (diversity), preventing accessibility to the RAG complex and recombination of the second allele, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, Catalytic component of the RAG complex
PurificationPurified by Protein A
Storage conditions 50% glycerol and 0, Keep refrigerated at 2 to 8 degrees Celcius for up to one year, 09% sodium azide, Store this antibody in aqueous buffered solution containing 1% BSA
Excitation emission650nm/665nm
Properties ALEXA FLUOR made this Alexa Fluor 633 conjugate that can be used in multi-color flow cytometry with instruments equipped with a second red laser or red diode, ALEXA FLUOR they should be stored frozen at - 24°, Alexa Fluor 633 is a practical alternative to APC as well as Cy5, Bioss Primary Conjugated Antibodies, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies, It is detected in the FL4 detector of the core's upgraded 2-laser FACScans, Like other Alexa Fluor dyes, exhibits uncommon photo stability, making it an ideal choice for fluorescent microscopy, the RAG2 Antibody, C, C for long term storage and for short term at + 5°, For facs or microscopy Alexa 1 conjugate
Conjugation ALEXA FLUOR 647, Alexa Fluor
Gene targetRAG2
Short nameRAG2 Antibody
Technique antibodies against human proteins, antibodies for, Antibody
LabelALEXA FLUOR 647
Alternative name ALEXA FLUOR 647, recombination activating gene 2 (Antibody to)
Alternative techniqueantibodies
Alternative to gene target RAG-2, RAG2 and IDBG-40186 and ENSG00000175097 and 5897, RAG2 and IDBG-636766 and ENSBTAG00000031309 and 782387, Rag2 and IDBG-193167 and ENSMUSG00000032864 and 19374, nuclei, phosphatidylinositol-4, this GO :0002326 and B cell lineage commitment and biological process this GO :0002331 and pre-B cell allelic exclusion and biological process this GO :0002358 and B cell homeostatic proliferation and biological process this GO :0002360 and T cell lineage commitment and biological process this GO :0003677 and DNA binding and molecular function this GO :0003682 and chromatin binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005546 and phosphatidylinositol-4, this GO :0003677: DNA binding, this GO :0003677: DNA binding and also this GO :0003682: chromatin binding and also this GO :0005515: protein binding and also this GO :0005546: phosphatidylinositol-4, this GO :0003682: chromatin binding, this GO :0005515: protein binding, this GO :0005546: phosphatidylinositol-4, this GO :0005547: phosphatidylinositol-3, this GO :0008270: zinc ion binding, this GO :0035064: methylated histone binding, this GO :0035091: phosphatidylinositol binding, this GO :0043325: phosphatidylinositol-3, this GO :0080025: phosphatidylinositol-3, 4, 4, 4, 4-bisphosphate binding, 4-bisphosphate binding and also this GO :0080025: phosphatidylinositol-3, 4-bisphosphate binding and molecular function this GO :0046622 and positive regulation of organ growth and biological process this GO :0080025 and phosphatidylinositol-3, 5-bisphosphate binding, 5-bisphosphate binding, 5-bisphosphate binding, 5-bisphosphate binding and also this GO :0005547: phosphatidylinositol-3, 5-bisphosphate binding and molecular function, 5-bisphosphate binding and molecular function this GO :0005547 and phosphatidylinositol-3, 5-trisphosphate binding, 5-trisphosphate binding and also this GO :0008270: zinc ion binding and also this GO :0035064: methylated histone binding and also this GO :0035091: phosphatidylinositol binding and also this GO :0043325: phosphatidylinositol-3, 5-trisphosphate binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0006310 and DNA recombination and biological process this GO :0008270 and zinc ion binding and molecular function this GO :0016568 and chromatin modification and biological process this GO :0030183 and B cell differentiation and biological process this GO :0030217 and T cell differentiation and biological process this GO :0033077 and T cell differentiation in thymus and biological process this GO :0033151 and V(D)J recombination and biological process this GO :0035064 and methylated histone binding and molecular function this GO :0035091 and phosphatidylinositol binding and molecular function this GO :0043325 and phosphatidylinositol-3, recombination activating gene 2
Identity 9832
Gene RAG2
Long gene name recombination activating 2
Synonyms gene name recombination activating gene 2
Locus 11p13
Discovery year 1990-08-15
GenBank acession AF080577
Entrez gene record 5897
Pubmed identfication 1283330
RefSeq identity NM_000536
Havana BLAST/BLAT OTTHUMG00000166393
Locus Specific Databases RAG2base: Mutation registry for autosomal recessive RAG2 deficiency LRG_99

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