RAG2 Antibody, HRP Conjugated

RAG2 Antibody, HRP Conjugated size: 0.1ml 350

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1

Price 350

Size 0.1ml

Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit

Long name	HRP Conjugated, RAG2 Polyclonal Antibody
Category	Conjugated Primary Antibodies
Conjugation	HRP Conjugated
Host Organism	Rabbit (Oryctolagus cuniculus)
Target Antigen	RAG2
Specificity	This is a highly specific antibody against RAG2
Modification	Unmodified
Modification site	None
Clonality	Polyclonal
Clone	Polyclonal antibody
Concentration	1ug per 1ul
Source	KLH conjugated synthetic peptide derived from human RAG2
Gene ID number	5897
Tested applications	IHC-P, WB
Recommended dilutions	IHC-P(1:100-500), WB(1:100-1000)
Crossreactivity	Mouse, Rat, Human
Crossreactive species details	not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background	5'-phosphorylated ends, DNA cleavage occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, Histone H3 monoubiquitination is required for the joining step of V(D)J recombination, In addition to its endonuclease activity, In the RAG complex, RAG1 also acts as a E3 ubiquitin-protein ligase that mediates monoubiquitination of histone H3, RAG1 mediates the DNA-binding to the conserved recombination signal sequences (RSS) and catalyzes the DNA cleavage activities by introducing a double-strand break between the RSS and the adjacent coding segment, RAG2 is not a catalytic component but is required for all known catalytic activities, The RAG complex also plays a role in pre-B cell allelic exclusion, The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps, The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T lymphocytes through rearrangement of different V (variable), a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B lymphocytes, and J (joining) gene segments, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, in some cases D (diversity), preventing accessibility to the RAG complex and recombination of the second allele, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, Catalytic component of the RAG complex
Purification method	This antibody was purified via Protein A
Storage conditions	50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% Gentamicin, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission	N/A
Properties	Also monoclonal HRP conjugates are often used for Western Blot, HRP conjugates are often primary rabbit polyclonal antibodies couples to the , found in the roots of , (HRP), C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°, enzyme , horseradish, horseradish peroxidase
Gene target	RAG2 Conjugated
Short name	Conjugated, RAG2 Antibody
Technique	antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
Label	HRP
Alternative name	horseradish peroxidase coupled, recombination activating gene 2 (Antibody to)
Alternative technique	antibodies
Alternative to gene target	RAG-2, RAG2 and IDBG-40186 and ENSG00000175097 and 5897, RAG2 and IDBG-636766 and ENSBTAG00000031309 and 782387, Rag2 and IDBG-193167 and ENSMUSG00000032864 and 19374, nuclei, phosphatidylinositol-4, this GO :0002326 and B cell lineage commitment and biological process this GO :0002331 and pre-B cell allelic exclusion and biological process this GO :0002358 and B cell homeostatic proliferation and biological process this GO :0002360 and T cell lineage commitment and biological process this GO :0003677 and DNA binding and molecular function this GO :0003682 and chromatin binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005546 and phosphatidylinositol-4, this GO :0003677: DNA binding, this GO :0003677: DNA binding and also this GO :0003682: chromatin binding and also this GO :0005515: protein binding and also this GO :0005546: phosphatidylinositol-4, this GO :0003682: chromatin binding, this GO :0005515: protein binding, this GO :0005546: phosphatidylinositol-4, this GO :0005547: phosphatidylinositol-3, this GO :0008270: zinc ion binding, this GO :0035064: methylated histone binding, this GO :0035091: phosphatidylinositol binding, this GO :0043325: phosphatidylinositol-3, this GO :0080025: phosphatidylinositol-3, 4, 4, 4, 4-bisphosphate binding, 4-bisphosphate binding and also this GO :0080025: phosphatidylinositol-3, 4-bisphosphate binding and molecular function this GO :0046622 and positive regulation of organ growth and biological process this GO :0080025 and phosphatidylinositol-3, 5-bisphosphate binding, 5-bisphosphate binding, 5-bisphosphate binding, 5-bisphosphate binding and also this GO :0005547: phosphatidylinositol-3, 5-bisphosphate binding and molecular function, 5-bisphosphate binding and molecular function this GO :0005547 and phosphatidylinositol-3, 5-trisphosphate binding, 5-trisphosphate binding and also this GO :0008270: zinc ion binding and also this GO :0035064: methylated histone binding and also this GO :0035091: phosphatidylinositol binding and also this GO :0043325: phosphatidylinositol-3, 5-trisphosphate binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0006310 and DNA recombination and biological process this GO :0008270 and zinc ion binding and molecular function this GO :0016568 and chromatin modification and biological process this GO :0030183 and B cell differentiation and biological process this GO :0030217 and T cell differentiation and biological process this GO :0033077 and T cell differentiation in thymus and biological process this GO :0033151 and V(D)J recombination and biological process this GO :0035064 and methylated histone binding and molecular function this GO :0035091 and phosphatidylinositol binding and molecular function this GO :0043325 and phosphatidylinositol-3, recombination activating gene 2
Identity	9832
Gene	RAG2
Long gene name	recombination activating 2
Synonyms gene name	recombination activating gene 2
Locus	11p13
Discovery year	1990-08-15
GenBank acession	AF080577
Entrez gene record	5897
Pubmed identfication	1283330
RefSeq identity	NM_000536
Havana BLAST/BLAT	OTTHUMG00000166393
Locus Specific Databases	RAG2base: Mutation registry for autosomal recessive RAG2 deficiency LRG_99

RAG2 Antibody, HRP Conjugated

RAG2 Antibody, HRP Conjugated size: 0.1ml 350

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