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RAPAd® Universal Adenoviral Expression System

RAPAd® Universal Adenoviral Expression System size: 1 test kit 1155

Price 1155
Size 1 test kit
We send this reagant onBlue Ice
The temperatureerature for storage is-20º, C
SDS or safty data sheet 251, 252, 253, 254, 255, 256) GHS, more (VPK-250, pdf
Type of reagentViral Expression
Related techniques Adenoviral Expression, Adenoviral Expression Systems, Viral Expression
Use of the reagent Adenoviral Expression Systems produce recombinant adenovirus with substantially reduced wild-type virus, More recent technologies have shortened this time somewhat, Serial amplification of adenovirus produced using the RAPAd®, The RAPAd®, The RAPAd®, The system uses a backbone vector from which the 5' ITR, Universal Adenoviral Expression System (# VPK-250) contains a promoterless shuttle vector to allow you to insert the promoter of your choice along with your gene of interest , but still produce relatively high amounts of wild type (replication-competent) plaques, levels of which increase with serial amplification, packaging signal and E1 sequences have been removed, system does not significantly increase replication-competent adenovirus levels, while considerably shortening the production time to 2-3 weeks, Making an adenovirus with traditional recombinant methods takes 2-3 months and requires tedious plaque recombination, gene expression, to maximize 
Cellular use000-fold fewer wild type plaques than most other expression systems, Engineered to produce 100- to 10
Protocol informationGenerate high-titer virus in about 2-3 weeks compared to 2-3 months with other systems
Reagent propertiesPromoterless shuttle vector allows you to clone the optimal promoter with your gene of interest
Additional lab advice5 kb, Cloning capacity of shuttle vector = 7
Living modified organisme in this reagent or LMONot applicable
Species compatible toHumans
Use of this test kit or productGenetics lab
Antigena specific protein sequence
Buffer70, The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7
Molecular weightin kilo Danlto or kDa
This reagent is purified97
Gene target Universal Adenoviral Expression, RAPAd®
Short name Universal Adenoviral Expression System, RAPAd®
Alternative name Universal Adenoviral Expression System, RAPAd®

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