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RAT ANTI FITC:Biotin Antibody

RAT ANTI FITC:Biotin Antibody size: 500ug 569

Supplier MBS MONO
Price 569
Size 500ug
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Also known asFITC
Other namesN/A
CategoryAntibodies
SubcategoryMnoclonal antibodies
Gene nameFITC
Gene name synonimsN/A
Other gene namesN/A
ClonalityMonoclonal
Immunoglobulin isotypeIgM
CloneLO-FLUO-1
Host organismRat (Rattus norvegicus)
Species reactivity Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby, Chemical
Specificity and cross-reactivity Avidity against FITC conjugated BSA is 2 x 10<, Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, This antibody binds to both Free FITC and FITC conjugated to proteins, This particular antibody might cross react with speacies outside of the listed ones, and may be valuable for amplification of FITC based immunostaining, -1<, /sup>, /sup>, 10<, FITC This item reacts with fluorescein isothiocyanate, M<, sup>, sup>
Purification methodN/A
Form/AppearanceBiotin (Purified IgM conjugated to Biotin - liquid)
Concentration0 mg/ml, Isotype concentration 1
Storage and shipping If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use, Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided, Shelf Life: 18 months from date of dispatch, Storage in frost free freezers is not recommended, the antibody should be stored undiluted, Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term
Tested applications: ELISA (EIA), Flow Cytometry (FACS)
Properties Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, MBS mono FITC is highly sensitive to pH extremes, This MBS mono Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, When excited at 488 nanometers, alkaline phosphatase substrates or anti biotin conjugated antibodies, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by MBS mono they should be stored frozen at - 24°
Conjugation Anti-FITC Antibody, Biotinylated
Short nameRAT ANTI FITC: Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, Antibody
LabelFITC
Alternative nameRAT antibody to fluorecein:biotinilated (Antibody to)
Alternative techniqueantibodies

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