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RAT ANTI MOUSE CD68:FITC Antibody

RAT ANTI MOUSE CD68:FITC Antibody size: 50ug 315

Supplier MBS MONO
Price 315
Size 50ug
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Also known asCD68
Other names CD68 antigen, Macrosialin, N/A, macrosialin, macrosialin
CategoryAntibodies
SubcategoryMnoclonal antibodies
Gene nameCD68
Gene name synonimsN/A
Other gene names Cd68, Lamp4, N/A, Scard1, gp110, Cd68
ClonalityMonoclonal
Immunoglobulin isotypeIgG2a
CloneFA-11
Host organismRat (Rattus norvegicus)
Species reactivity Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby, Mouse (Mus musculus)
Specificity and cross-reactivity Langerhans cells and at low levels by dendritic cells, Macrosialin is the murine homologue of the human macrophage glycoprotein CD68, Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, This item does detect surface macrosialin at low levels in resident mouse peritoneal macrophages which can be enhanced with thioglycollate stimulation, This particular antibody might cross react with speacies outside of the listed ones, both are members of the lysosomal-associated membrane protein (lamp) family which are located predominantly within the cells and can be detected by flow cytometry using cell permeabilisation, which is specifically expressed by tissue macrophages, CD68 Macrosialin is a heavily glycosylated transmembrane protein of 87-115kD
Purification methodN/A
Form/AppearanceFITC (Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid)
Concentration1 miligram per 1 mililiter, IgG concentration 0
Storage and shipping If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use, Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided, Shelf Life: 18 months from date of dispatch, Storage in frost free freezers is not recommended, the antibody is photosensitive and should be protected from light, the antibody should be stored undiluted, Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term
Tested applications:Flow Cytometry (FACS)
Properties FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, MBS mono FITC is highly sensitive to pH extremes, This MBS mono Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by MBS mono they should be stored frozen at - 24°
ConjugationAnti-FITC Antibody
Gene targetCD68:
Short nameRAT ANTI MOUSE CD68:FITC Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, Antibody
LabelFITC
Alternative nameRAT antibody to MOUSE CD68 molecule:fluorecein (Antibody to)
Alternative techniqueantibodies
Alternative to gene target CD68 and IDBG-25923 and ENSG00000129226 and 968, CD68 and IDBG-635034 and ENSBTAG00000000133 and 504960, Cd68 and IDBG-191443 and ENSMUSG00000018774 and 12514, GP110 and LAMP4 and SCARD1, Plasma membranes, this GO :0005765 and lysosomal membrane and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0010008 and endosome membrane and cellular component this GO :0016020 and membrane and cellular component this GO :0016021 and integral component of membrane and cellular component this GO :0071310 and cellular response to organic substance and biological process, CD68 molecule
Identity 1693
Gene CD68
Long gene name CD68 molecule
Synonyms gene name CD68 antigen
Synonyms SCARD1 macrosialin GP110 DKFZp686M18236 LAMP4
Synonyms name member 1 CD68 antigen macrophage antigen CD68 , scavenger receptor class D
Locus 17p13, 1
Discovery year 1993-06-11
GenBank acession S57235
Entrez gene record 968
Pubmed identfication 9790779
RefSeq identity NM_001251
Classification CD molecules Scavenger receptors
Havana BLAST/BLAT OTTHUMG00000108146

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