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Recombinant Human Cytoplasmic Protein NCK1 (N-6His)

Recombinant Human Cytoplasmic Protein NCK1 (N-6His) size: 1 mg 2283

Supplier NOVO
Price 2283
Size 1 mg
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Reacts withHuman
Source proteins, Recombinants or rec
DescriptionRecombinant Human NCK1 is produced by our E, coli expression system and the target gene encoding Met1-Ser377 is expressed with a 6His tag at the N-terminus
Molecular Weight45 kD
UniProt numberP16333
State of the productFreeze-dried
Shipping conditionsAmbient temperature
Formulation 50 mM sodium chloride, pH 8, 2 um filtered solution of 20 mM Tris, Lyophilized from a 0
Storage recommendations -20°, Aliquots of reconstituted samples are stable at less -20°, If kept at at room temperature, If kept at at room temperature, Reconstituted protein solution should be stored at 4-7°, it is stable for 3 weeks, it is stable for 3 weeks, C, C for 2-7 days, C for 3 months, Freeze-dried proteins should be stored frozen at <
Reconstitution Dissolve the lyophilized protein in ddH2O, Do not mix by vortex or pipetting, It is not recommended to reconstitute to a concentration less than 100 &mu, Please aliquot the reconstituted solution to minimize freeze-thaw cycles, Always centrifuge tubes before opening, g/ml
Purity and determined by Polyacrylamide gel electrophoresis, Greater than 95%
Sequence of the amino acidMGSSHHHHHHSSGLVPRGSHMAEEVVVVAKFDYVAQQEQELDIKKNERLWLLDDSKSWWRVRNSMNKTGFVPSNYVERKNSARKASIVKNLKDTLGIGKVKRKPSVPDSASPADDSFVDPGERLYDLNMPAYVKFNYMAEREDELSLIKGTKVIVMEKCSDGWWRGSYNGQVGWFPSNYVTEEGDSPLGDHVGSLSEKLAAVVNNLNTGQVLHVVQALYPFSSSNDEELNFEKGDVMDVIEKPENDPEWWKCRKINGMVGLVPKNYVTVMQNNPLTSGLEPSPPQCDYIRPSLTGKFAGNPWYYGKVTRHQAEMALNERGHEGDFLIRDSESSPNDFSVSLKAQGKNKHFKVQLKETVYCIGQRKFSTMEELVEHYKKAPIFTSEQGEKLYLVKHLS
Levels of endotoxin11 IEU/ug, LAL test shows less than than 0
Properties Depending on the epitopes used human ELISA kits can be cross reactive to many other species, Mainly analyzed are human serum, Modern , cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies, human cell culture supernatants and biological samples, plasma, primarily , saliva, urine,  , (Homo sapiens, Homo sapiens sapiens), Human proteins, humans , ssp
Grouprecombinants
Gene targetCytoplasmic Protein NCK1 (N-6His)
Short nameRecombinant Cytoplasmic Protein NCK1 (N-6His)
Technique E, coli recombinant proteins are , novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture, supplied as white sterile powder lyopillized, Recombinant, genetic recombinations , in Escherichia coli
Species Humans, Human
Alternative name sapiens Cytoplasmic Protein NCK adaptor protein 1 (N-6His), recombinant H
Alternative techniquerec
Alternative to gene target NCK and nck-1 and NCKalpha, NCK1 and IDBG-58017 and ENSG00000158092 and 4690, NCK1 and IDBG-638662 and ENSBTAG00000018427 and 536154, Nck1 and IDBG-193200 and ENSMUSG00000032475 and 17973, nuclei, receptor tyrosine kinase binding, this GO :0004860 and protein kinase inhibitor activity and molecular function this GO :0005102 and receptor binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005783 and endoplasmic reticulum and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005911 and cell-cell junction and cellular component this GO :0006417 and regulation of translation and biological process this GO :0006469 and negative regulation of protein kinase activity and biological process this GO :0007015 and actin filament organization and biological process this GO :0007172 and signal complex assembly and biological process this GO :0007411 and axon guidance and biological process this GO :0008093 and cytoskeletal adaptor activity and molecular function this GO :0012506 and vesicle membrane and cellular component this GO :0016477 and cell migration and biological process this GO :0019887 and protein kinase regulator activity and molecular function this GO :0019904 and protein domain specific binding and molecular function this GO :0030032 and lamellipodium assembly and biological process this GO :0030159 and receptor signaling complex scaffold activity and molecular function this GO :0030838 and positive regulation of actin filament polymerization and biological process this GO :0030971 and receptor tyrosine kinase binding and molecular function this GO :0038096 and Fc-gamma receptor signaling pathway involved in pha this GO cytosis and biological process this GO :0042102 and positive regulation of T cell proliferation and biological process this GO :0042110 and T cell activation and biological process this GO :0045087 and innate immune response and biological process this GO :0045859 and regulation of protein kinase activity and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0050852 and T cell receptor signaling pathway and biological process this GO :0051707 and response to other organism and biological process this GO :0060548 and negative regulation of cell death and biological process, this GO :0004860: protein kinase inhibitor activity, this GO :0004860: protein kinase inhibitor activity and also this GO :0005102: receptor binding and also this GO :0005515: protein binding and also this GO :0008093: cytoskeletal adaptor activity and also this GO :0019887: protein kinase regulator activity and also this GO :0019904: protein domain specific binding and also this GO :0030159: receptor signaling complex scaffold activity and also this GO :0030971: receptor tyrosine kinase binding, this GO :0005102: receptor binding, this GO :0005515: protein binding, this GO :0008093: cytoskeletal adaptor activity, this GO :0019887: protein kinase regulator activity, this GO :0019904: protein domain specific binding, this GO :0030159: receptor signaling complex scaffold activity, this GO :0030971: receptor tyrosine kinase binding, NCK adaptor protein 1
Identity 7664
Gene NCK1
Long gene name NCK adaptor protein 1
Synonyms gene NCK
Synonyms NCKalpha
Locus 3q22, 3
Discovery year 1998-11-04
GenBank acession X17576
Entrez gene record 4690
Pubmed identfication 7806213 9737977
RefSeq identity NM_006153
Classification SH2 domain containing
Havana BLAST/BLAT OTTHUMG00000159781

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