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Recombinant Human Lysine--tRNA Ligase, KARS (C-6His)

Recombinant Human Lysine--tRNA Ligase, KARS (C-6His) size: 50 µg 496

Supplier NOVO
Price 496
Size 50 µg
Reacts withHuman
Source proteins, Recombinants or rec
DescriptionRecombinant Human Lysine--tRNA Ligase is produced by our Mammalian expression system and the target gene encoding Ala2-Val597 is expressed with a 6His tag at the C-terminus
Molecular Weight1 kD, 69
UniProt numberQ15046
State of the productLiquid
Shipping conditionsDry Ice/ice packs
Formulation1 mM DTT, 150 mM sodium chloride, 2 um filtered solution of 20 mM TrisHCl, 20% glycerol, Supplied as a 0, pH8
Storage recommendations -20°, Please minimize freeze-thaw cycles, stable for 6 months after receipt, C, Store at <
Reconstitution Dissolve the lyophilized protein in ddH2O, Do not mix by vortex or pipetting, It is not recommended to reconstitute to a concentration less than 100 &mu, Please aliquot the reconstituted solution to minimize freeze-thaw cycles, Always centrifuge tubes before opening, g/ml
Purity and determined by Polyacrylamide gel electrophoresis, Greater than 95%
Sequence of the amino acidAAVQAAEVKVDGSEPKLSKNELKRRLKAEKKVAEKEAKQKELSEKQLSQATAAATNHTTDNGVGPEEESVDPNQYYKIRSQAIHQLKVNGEDPYPHKFHVDISLTDFIQKYSHLQPGDHLTDITLKVAGRIHAKRASGGKLIFYDLRGEGVKLQVMANSRNYKSEEEFIHINNKLRRGDIIGVQGNPGKTKKGELSIIPYEITLLSPCLHMLPHLHFGLKDKETRYRQRYLDLILNDFVRQKFIIRSKIITYIRSFLDELGFLEIETPMMNIIPGGAVAKPFITYHNELDMNLYMRIAPELYHKMLVVGGIDRVYEIGRQFRNEGIDLTHNPEFTTCEFYMAYADYHDLMEITEKMVSGMVKHITGSYKVTYHPDGPEGQAYDVDFTPPFRRINMVEELEKALGMKLPETNLFETEETRKILDDICVAKAVECPPPRTTARLLDKLVGEFLEVTCINPTFICDHPQIMSPLAKWHRSKEGLTERFELFVMKKEICNAYTELNDPMRQRQLFEEQAKAKAAGDDEAMFIDENFCTALEYGLPPTAGWGMGIDRVAMFLTDSNNIKEVLLFPAMKPEDKKENVATTDTLESTTVGTSVVDHHHHHH
Levels of endotoxin11 IEU/ug, LAL test shows less than than 0
Properties Depending on the epitopes used human ELISA kits can be cross reactive to many other species, Mainly analyzed are human serum, Modern , cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies, human cell culture supernatants and biological samples, plasma, primarily , saliva, urine,  , (Homo sapiens, Homo sapiens sapiens), Human proteins, humans , ssp
Grouprecombinants
Gene target KARS (C-6His), Lysine tRNA Ligase
Short name KARS (C-6His), Recombinant Lysine--tRNA Ligase
Technique E, coli recombinant proteins are , novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture, supplied as white sterile powder lyopillized, Recombinant, genetic recombinations , in Escherichia coli
Species Humans, Human
Alternative name KARS (C-6His), sapiens Lysine--tRNA Ligase, recombinant H
Alternative techniquerec
Identity 6215
Gene KARS
Long gene name lysyl-tRNA synthetase
Synonyms gene DFNB89
Synonyms gene name autosomal recessive 89 , deafness
Synonyms KARS2 KARS1
Synonyms name lysine tRNA ligase
Locus 16q23, 1
Discovery year 1995-07-11
GenBank acession AF285758
Entrez gene record 3735
Pubmed identfication 8812440 9278442 23768514
RefSeq identity NM_005548
Classification Aminoacyl tRNA synthetases, Class II Deafness associated genes
Havana BLAST/BLAT OTTHUMG00000137609
Locus Specific Databases LRG_366

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