Name : Recombinant Human Pyruvate Kinase, Liver And RBC, PKLR (C-6His)
Supplier : NOVO
Price :156
SKU : GEN2123841809
| Reacts with | Human |
| Source | proteins, Recombinants or rec |
| Description | Recombinant Human PKLR is produced by our Mammalian expression system and the target gene encoding Met1-Ser574 is expressed with a 6His tag at the C-terminus |
| Molecular Weight | 62, 9 kD |
| UniProt number | P30613 |
| State of the product | Liquid |
| Shipping conditions | Dry ice/ice packs |
| Formulation | 1 mM DTT 0, 10% glycerol, pH 8, 2 um filtered solution of 20 mM Tris-HCl, 2M sodium chloride, Supplied as a 0 |
| Storage recommendations | -20°, Please minimize freeze-thaw cycles, stable for 6 months after receipt, C, Store at < |
| Reconstitution | Dissolve the lyophilized protein in ddH2O, Do not mix by vortex or pipetting, It is not recommended to reconstitute to a concentration less than 100 &mu, Please aliquot the reconstituted solution to minimize freeze-thaw cycles, Always centrifuge tubes before opening, g/ml |
| Purity | and determined by Polyacrylamide gel electrophoresis, Greater than 95% |
| Sequence of the amino acid | MSIQENISSLQLRSWVSKSQRDLAKSILIGAPGGPAGYLRRASVAQLTQELGTAFFQQQQLPAAMADTFLEHLCLLDIDSEPVAARSTSIIATIGPASRSVERLKEMIKAGMNIARLNFSHGSHEYHAESIANVREAVESFAGSPLSYRPVAIALDTKGPEIRTGILQGGPESEVELVKGSQVLVTVDPAFRTRGNANTVWVDYPNIVRVVPVGGRIYIDDGLISLVVQKIGPEGLVTQVENGGVLGSRKGVNLPGAQVDLPGLSEQDVRDLRFGVEHGVDIVFASFVRKASDVAAVRAALGPEGHGIKIISKIENHEGVKRFDEILEVSDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNLAGKPVVCATQMLESMITKPRPTRAETSDVANAVLDGADCIMLSGETAKGNFPVEAVKMQHAIAREAEAAVYHRQLFEELRRAAPLSRDPTEVTAIGAVEAAFKCCAAAIIVLTTTGRSAQLLSRYRPRAAVIAVTRSAQAARQVHLCRGVFPLLYREPPEAIWADDVDRRVQFGIESGKLRGFLRVGDLVIVVTGWRPGSGYTNIMRVLSISVDHHHHHH |
| Levels of endotoxin | 11 IEU/ug, LAL test shows less than than 0 |
| Properties | Depending on the epitopes used human ELISA kits can be cross reactive to many other species, Mainly analyzed are human serum, Modern , cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies, human cell culture supernatants and biological samples, plasma, primarily , saliva, urine,  , (Homo sapiens, Homo sapiens sapiens), Human proteins, humans , ssp |
| Group | recombinants |
| Gene target | Liver RBC, PKLR (C-6His), Pyruvate Kinase |
| Short name | Liver And RBC, PKLR (C-6His), Recombinant Pyruvate Kinase |
| Technique | E, coli recombinant proteins are , novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture, supplied as white sterile powder lyopillized, Recombinant, genetic recombinations , in Escherichia coli |
| Species | Humans, Human |
| Alternative name | Liver And RBC, PKLR (C-6His), sapiens Pyruvate phosphorylation catalyst, recombinant H |
| Alternative technique | rec |
| Identity | 9020 |
| Gene | PKLR |
| Long gene name | liver and RBC , pyruvate kinase |
| Locus | 1q22 |
| Discovery year | 2001-06-22 |
| GenBank acession | BC025737 M15465 |
| Entrez gene record | 5313 |
| Pubmed identfication | 3566732 |
| RefSeq identity | NM_000298 |
| Havana BLAST/BLAT | OTTHUMG00000035875 |