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Recombinant Human Pyruvate Kinase M2, PKM2 (N-6His)

Recombinant Human Pyruvate Kinase M2, PKM2 (N-6His) size: 10 µg 121

Supplier NOVO
Price 121
Size 10 µg
Reacts withHuman
Source proteins, Recombinants or rec
DescriptionRecombinant Human Pyruvate kinase M2 is produced by our E, coli expression system and the target gene encoding Ser2-Pro531 is expressed with a 6His tag at the N-terminus
Molecular Weight1 kD, 60
UniProt numberP14618
State of the productLiquid
Shipping conditionsDry ice/ice packs
Formulation 10% glycerol, pH7, 2 um filtered solution of phosphate buffered saline, Supplied as a 0
Storage recommendations -20°, Please minimize freeze-thaw cycles, stable for 6 months after receipt, C, Store at <
Reconstitution Dissolve the lyophilized protein in ddH2O, Do not mix by vortex or pipetting, It is not recommended to reconstitute to a concentration less than 100 &mu, Please aliquot the reconstituted solution to minimize freeze-thaw cycles, Always centrifuge tubes before opening, g/ml
Purity and determined by Polyacrylamide gel electrophoresis, Greater than 95%
Sequence of the amino acidMGSSHHHHHHSSGLVPRGSHMSKPHSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSPPITARNTGIICTIGPASRSVETLKEMIKSGMNVARLNFSHGTHEYHAETIKNVRTATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKQKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKASDVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAIYHLQLFEELRRLAPITSDPTEATAVGAVEASFKCCSGAIIVLTKSGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDPVQEAWAEDVDLRVNFAMNVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPVP
Levels of endotoxin11 IEU/ug, LAL test shows less than than 0
Properties Depending on the epitopes used human ELISA kits can be cross reactive to many other species, Mainly analyzed are human serum, Modern , cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies, human cell culture supernatants and biological samples, plasma, primarily , saliva, urine,  , (Homo sapiens, Homo sapiens sapiens), Human proteins, humans , ssp
Grouprecombinants
Gene target PKM2 (N-6His), Pyruvate Kinase M2
Short name PKM2 (N-6His), Recombinant Pyruvate Kinase M2
Technique E, coli recombinant proteins are , novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture, supplied as white sterile powder lyopillized, Recombinant, genetic recombinations , in Escherichia coli
Species Humans, Human
Alternative name muscle (N-6His), pyruvate kinase, sapiens Pyruvate phosphorylation catalyst M2, recombinant H
Alternative techniquerec
Alternative to gene target CTHBP and HEL-S-30 and OIP3 and PK3 and PKM2 and TCB and THBP1, PKM2 and IDBG-20117 and ENSG00000067225 and 5315, PKM2 and IDBG-645572 and ENSBTAG00000001601 and 512571, Pkm and IDBG-173276 and ENSMUSG00000032294 and 18746, muscle, nuclei, poly(A) RNA binding, this GO :0000287 and magnesium ion binding and molecular function this GO :0003824 and catalytic activity and molecular function this GO :0004743 and pyruvate kinase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005739 and mitochondrion and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005929 and cilium and cellular component this GO :0005975 and carbohydrate metabolic process and biological process this GO :0006006 and glucose metabolic process and biological process this GO :0006096 and glycolytic process and biological process this GO :0012501 and programmed cell death and biological process this GO :0016310 and phosphorylation and biological process this GO :0023026 and MHC class II protein complex binding and molecular function this GO :0030955 and potassium ion binding and molecular function this GO :0044281 and small molecule metabolic process and biological process this GO :0044822 and poly(A) RNA binding and molecular function this GO :0070062 and extracellular vesicular exosome and cellular component, this GO :0000287: magnesium ion binding, this GO :0000287: magnesium ion binding and also this GO :0003824: catalytic activity and also this GO :0004743: pyruvate kinase activity and also this GO :0005515: protein binding and also this GO :0005524: ATP binding and also this GO :0023026: MHC class II protein complex binding and also this GO :0030955: potassium ion binding and also this GO :0044822: poly(A) RNA binding, this GO :0003824: catalytic activity, this GO :0004743: pyruvate kinase activity, this GO :0005515: protein binding, this GO :0005524: ATP binding, this GO :0023026: MHC class II protein complex binding, this GO :0030955: potassium ion binding, this GO :0044822: poly(A) RNA binding, pyruvate kinase
Identity 9021
Gene PKM
Long gene name muscle , pyruvate kinase
Synonyms gene PKM2
Synonyms THBP1 OIP3 PK3
Locus 15q23
Discovery year 2001-06-22
GenBank acession M23725
Entrez gene record 5315
Pubmed identfication 2040271
Havana BLAST/BLAT OTTHUMG00000172709

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