Name : Recombinant Human Pyruvate Kinase M2, PKM2 (N-6His)
Supplier : NOVO
Price :121
SKU : GEN8879094222
| Reacts with | Human |
| Source | proteins, Recombinants or rec |
| Description | Recombinant Human Pyruvate kinase M2 is produced by our E, coli expression system and the target gene encoding Ser2-Pro531 is expressed with a 6His tag at the N-terminus |
| Molecular Weight | 1 kD, 60 |
| UniProt number | P14618 |
| State of the product | Liquid |
| Shipping conditions | Dry ice/ice packs |
| Formulation | 10% glycerol, pH7, 2 um filtered solution of phosphate buffered saline, Supplied as a 0 |
| Storage recommendations | -20°, Please minimize freeze-thaw cycles, stable for 6 months after receipt, C, Store at < |
| Reconstitution | Dissolve the lyophilized protein in ddH2O, Do not mix by vortex or pipetting, It is not recommended to reconstitute to a concentration less than 100 &mu, Please aliquot the reconstituted solution to minimize freeze-thaw cycles, Always centrifuge tubes before opening, g/ml |
| Purity | and determined by Polyacrylamide gel electrophoresis, Greater than 95% |
| Sequence of the amino acid | MGSSHHHHHHSSGLVPRGSHMSKPHSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSPPITARNTGIICTIGPASRSVETLKEMIKSGMNVARLNFSHGTHEYHAETIKNVRTATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKQKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKASDVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAIYHLQLFEELRRLAPITSDPTEATAVGAVEASFKCCSGAIIVLTKSGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDPVQEAWAEDVDLRVNFAMNVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPVP |
| Levels of endotoxin | 11 IEU/ug, LAL test shows less than than 0 |
| Properties | Depending on the epitopes used human ELISA kits can be cross reactive to many other species, Mainly analyzed are human serum, Modern , cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies, human cell culture supernatants and biological samples, plasma, primarily , saliva, urine,  , (Homo sapiens, Homo sapiens sapiens), Human proteins, humans , ssp |
| Group | recombinants |
| Gene target | PKM2 (N-6His), Pyruvate Kinase M2 |
| Short name | PKM2 (N-6His), Recombinant Pyruvate Kinase M2 |
| Technique | E, coli recombinant proteins are , novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture, supplied as white sterile powder lyopillized, Recombinant, genetic recombinations , in Escherichia coli |
| Species | Humans, Human |
| Alternative name | muscle (N-6His), pyruvate kinase, sapiens Pyruvate phosphorylation catalyst M2, recombinant H |
| Alternative technique | rec |
| Alternative to gene target | CTHBP and HEL-S-30 and OIP3 and PK3 and PKM2 and TCB and THBP1, PKM2 and IDBG-20117 and ENSG00000067225 and 5315, PKM2 and IDBG-645572 and ENSBTAG00000001601 and 512571, Pkm and IDBG-173276 and ENSMUSG00000032294 and 18746, muscle, nuclei, poly(A) RNA binding, this GO :0000287 and magnesium ion binding and molecular function this GO :0003824 and catalytic activity and molecular function this GO :0004743 and pyruvate kinase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005739 and mitochondrion and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005929 and cilium and cellular component this GO :0005975 and carbohydrate metabolic process and biological process this GO :0006006 and glucose metabolic process and biological process this GO :0006096 and glycolytic process and biological process this GO :0012501 and programmed cell death and biological process this GO :0016310 and phosphorylation and biological process this GO :0023026 and MHC class II protein complex binding and molecular function this GO :0030955 and potassium ion binding and molecular function this GO :0044281 and small molecule metabolic process and biological process this GO :0044822 and poly(A) RNA binding and molecular function this GO :0070062 and extracellular vesicular exosome and cellular component, this GO :0000287: magnesium ion binding, this GO :0000287: magnesium ion binding and also this GO :0003824: catalytic activity and also this GO :0004743: pyruvate kinase activity and also this GO :0005515: protein binding and also this GO :0005524: ATP binding and also this GO :0023026: MHC class II protein complex binding and also this GO :0030955: potassium ion binding and also this GO :0044822: poly(A) RNA binding, this GO :0003824: catalytic activity, this GO :0004743: pyruvate kinase activity, this GO :0005515: protein binding, this GO :0005524: ATP binding, this GO :0023026: MHC class II protein complex binding, this GO :0030955: potassium ion binding, this GO :0044822: poly(A) RNA binding, pyruvate kinase |
| Identity | 9021 |
| Gene | PKM |
| Long gene name | muscle , pyruvate kinase |
| Synonyms gene | PKM2 |
| Synonyms | THBP1 OIP3 PK3 |
| Locus | 15q23 |
| Discovery year | 2001-06-22 |
| GenBank acession | M23725 |
| Entrez gene record | 5315 |
| Pubmed identfication | 2040271 |
| Havana BLAST/BLAT | OTTHUMG00000172709 |