Name : Recombinant Human Semaphorin-4D, SEMA4D, CD100 (C-6His)
Supplier : NOVO
Price :2283
SKU : GEN8487104292
| Reacts with | Human |
| Source | proteins, Recombinants or rec |
| Description | Recombinant Human Semaphorin 4D is produced by our Mammalian expression system and the target gene encoding Met22-Arg734 is expressed with a 6His tag at the C-terminus |
| Molecular Weight | 2 kD, 80 |
| UniProt number | Q92854 |
| State of the product | Freeze-dried |
| Shipping conditions | Ambient temperature |
| Formulation | pH 7, 150 mM sodium chloride, 2 um filtered solution of 20 mM PB, 4, Lyophilized from a 0 |
| Storage recommendations | -20°, Aliquots of reconstituted samples are stable at less -20°, If kept at at room temperature, If kept at at room temperature, Reconstituted protein solution should be stored at 4-7°, it is stable for 3 weeks, it is stable for 3 weeks, C, C for 2-7 days, C for 3 months, Freeze-dried proteins should be stored frozen at < |
| Reconstitution | Dissolve the lyophilized protein in ddH2O, Do not mix by vortex or pipetting, It is not recommended to reconstitute to a concentration less than 100 &mu, Please aliquot the reconstituted solution to minimize freeze-thaw cycles, Always centrifuge tubes before opening, g/ml |
| Purity | and determined by Polyacrylamide gel electrophoresis, Greater than 95% |
| Sequence of the amino acid | MAFAPIPRITWEHREVHLVQFHEPDIYNYSALLLSEDKDTLYIGAREAVFAVNALNISEKQHEVYWKVSEDKKAKCAEKGKSKQTECLNYIRVLQPLSATSLYVCGTNAFQPACDHLNLTSFKFLGKNEDGKGRCPFDPAHSYTSVMVDGELYSGTSYNFLGSEPIISRNSSHSPLRTEYAIPWLNEPSFVFADVIRKSPDSPDGEDDRVYFFFTEVSVEYEFVFRVLIPRIARVCKGDQGGLRTLQKKWTSFLKARLICSRPDSGLVFNVLRDVFVLRSPGLKVPVFYALFTPQLNNVGLSAVCAYNLSTAEEVFSHGKYMQSTTVEQSHTKWVRYNGPVPKPRPGACIDSEARAANYTSSLNLPDKTLQFVKDHPLMDDSVTPIDNRPRLIKKDVNYTQIVVDRTQALDGTVYDVMFVSTDRGALHKAISLEHAVHIIEETQLFQDFEPVQTLLLSSKKGNRFVYAGSNSGVVQAPLAFCGKHGTCEDCVLARDPYCAWSPPTATCVALHQTESPSRGLIQEMSGDASVCPDKSKGSYRQHFFKHGGTAELKCSQKSNLARVFWKFQNDVLKAESPKYGLMGRKNLLIFNLSEGDSGVYQCLSEERVKNKTVFQVVAKHVLEVKVVPKPVVAPTLSVVQTEGSRIATKVLVASTQGSSPPTPAVQATSSGAITLPPKPAPTGTSCEPKIVINTVPQLHSEKTMYLKSSDNRVDHHHHHH |
| Levels of endotoxin | 11 IEU/ug, LAL test shows less than than 0 |
| Properties | Depending on the epitopes used human ELISA kits can be cross reactive to many other species, Mainly analyzed are human serum, Modern , cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies, human cell culture supernatants and biological samples, plasma, primarily , saliva, urine,  , (Homo sapiens, Homo sapiens sapiens), Human proteins, humans , ssp |
| Group | recombinants |
| Gene target | CD100 (C-6His), SEMA4D, Semaphorin-4D |
| Short name | CD100 (C-6His), SEMA4D, Recombinant Semaphorin-4D |
| Technique | E, coli recombinant proteins are , novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture, supplied as white sterile powder lyopillized, Recombinant, genetic recombinations , in Escherichia coli |
| Species | Humans, Human |
| Alternative name | (semaphorin) 4D, CD100 (C-6His), immunoglobulin domain (Ig), sapiens Semaphorin-4D, sema domain, transmembrane domain (TM) and short cytoplasmic domain, recombinant H |
| Alternative technique | rec |
| Alternative to gene target | (semaphorin) 4D, C9orf164 and CD100 and coll-4 and M-sema-G and SEMAJ, Extracellular, SEMA4D and IDBG-637043 and ENSBTAG00000001941 and 785942, SEMA4D and IDBG-75424 and ENSG00000187764 and 10507, Sema4d and IDBG-152500 and ENSMUSG00000021451 and 20354, immunoglobulin domain (Ig), semaphorin receptor binding, this GO :0000122 and negative regulation of transcription from RNA polymerase II promoter and biological process this GO :0001934 and positive regulation of protein phosphorylation and biological process this GO :0004872 and receptor activity and molecular function this GO :0004888 and transmembrane signaling receptor activity and molecular function this GO :0005102 and receptor binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005615 and extracellular space and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005887 and integral component of plasma membrane and cellular component this GO :0006955 and immune response and biological process this GO :0007155 and cell adhesion and biological process this GO :0007162 and negative regulation of cell adhesion and biological process this GO :0007275 and multicellular organismal development and biological process this GO :0007411 and axon guidance and biological process this GO :0008360 and regulation of cell shape and biological process this GO :0010693 and negative regulation of alkaline phosphatase activity and biological process this GO :0014068 and positive regulation of phosphatidylinositol 3-kinase signaling and biological process this GO :0016020 and membrane and cellular component this GO :0030215 and semaphorin receptor binding and molecular function this GO :0030335 and positive regulation of cell migration and biological process this GO :0031344 and regulation of cell projection organization and biological process this GO :0032321 and positive regulation of Rho GTPase activity and biological process this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0043931 and ossification involved in bone maturation and biological process this GO :0045668 and negative regulation of osteoblast differentiation and biological process this GO :0048672 and positive regulation of collateral sprouting and biological process this GO :0048814 and regulation of dendrite morphogenesis and biological process this GO :0050731 and positive regulation of peptidyl-tyrosine phosphorylation and biological process this GO :0050732 and negative regulation of peptidyl-tyrosine phosphorylation and biological process this GO :0050772 and positive regulation of axonogenesis and biological process this GO :0070486 and leukocyte aggregation and biological process this GO :0071526 and semaphorin-plexin signaling pathway and biological process this GO :1900220 and semaphorin-plexin signaling pathway involved in bone trabecula morphogenesis and biological process, this GO :0004872: receptor activity, this GO :0004872: receptor activity and also this GO :0004888: transmembrane signaling receptor activity and also this GO :0005102: receptor binding and also this GO :0005515: protein binding and also this GO :0030215: semaphorin receptor binding, this GO :0004888: transmembrane signaling receptor activity, this GO :0005102: receptor binding, this GO :0005515: protein binding, this GO :0030215: semaphorin receptor binding, transmembrane domain (TM) and short cytoplasmic domain, sema domain |
| Identity | 10732 |
| Gene | SEMA4D |
| Long gene name | semaphorin 4D |
| Synonyms gene | SEMAJ C9orf164 |
| Synonyms gene name | (semaphorin) 4D , chromosome 9 open reading frame 164 sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain |
| Synonyms | CD100 coll-4 FLJ39737 |
| Synonyms name | M-sema G |
| Locus | 9q22, 2 |
| Discovery year | 1999-06-25 |
| GenBank acession | U60800 |
| Entrez gene record | 10507 |
| Pubmed identfication | 8876214 8969198 |
| RefSeq identity | NM_006378 |
| Classification | CD molecules Semaphorins Immunoglobulin like domain containing |
| Havana BLAST/BLAT | OTTHUMG00000020185 |