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Recombinant Human Tumor Necrosis Factor α, TNFα (C-6His)

Recombinant Human Tumor Necrosis Factor α, TNFα (C-6His) size: 10 µg 95

Supplier NOVO
Price 95
Size 10 µg
Reacts withHuman
Source proteins, Recombinants or rec
DescriptionRecombinant Human Tumor Necrosis Factor alpha is produced by our E, coli expression system and the target gene encoding Val77-Leu233 is expressed with a 6His tag at the C-terminus
Molecular Weight18, 5 kD
UniProt numberP01375
State of the productFreeze-dried
Shipping conditionsAmbient temperature
Formulation2 um filtered solution of 20 mM PB,150 mM sodium chloride, 4, Lyophilized from a 0, pH7
Storage recommendations -20°, Aliquots of reconstituted samples are stable at less -20°, If kept at at room temperature, If kept at at room temperature, Reconstituted protein solution should be stored at 4-7°, it is stable for 3 weeks, it is stable for 3 weeks, C, C for 2-7 days, C for 3 months, Freeze-dried proteins should be stored frozen at <
Reconstitution Dissolve the lyophilized protein in ddH2O, Do not mix by vortex or pipetting, It is not recommended to reconstitute to a concentration less than 100 &mu, Please aliquot the reconstituted solution to minimize freeze-thaw cycles, Always centrifuge tubes before opening, g/ml
Purity and determined by Polyacrylamide gel electrophoresis, Greater than 95%
Sequence of the amino acidMVRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIALLEHHHHHH
Levels of endotoxin11 IEU/ug, LAL test shows less than than 0
Properties Depending on the epitopes used human ELISA kits can be cross reactive to many other species, Mainly analyzed are human serum, Modern , cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies, human cell culture supernatants and biological samples, plasma, primarily , saliva, urine,  , (Homo sapiens, Homo sapiens sapiens), Human proteins, humans , ssp
Grouprecombinants
Gene target (C-6His), TNF&alpha, Tumor Necrosis Factor &alpha
Short name (C-6His), TNF&alpha, Recombinant Tumor Necrosis Factor &alpha
Technique E, coli recombinant proteins are , novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture, supplied as white sterile powder lyopillized, Recombinant, genetic recombinations , in Escherichia coli
SpeciesHumans
Alternative name (C-6His), TNF&alpha, sapiens Tumor Necrosis Factor &alpha, recombinant H
Alternative techniquerec
Identity 11892
Gene TNF
Long gene name tumor necrosis factor
Synonyms gene TNFA
Synonyms gene name member 2) , tumor necrosis factor (TNF superfamily
Synonyms TNFSF2 DIF TNF-alpha
Synonyms name TNF superfamily, member 2
Locus 6p21, 33
Discovery year 1986-01-01
GenBank acession X02910
Entrez gene record 7124
Pubmed identfication 2413547 6392892
Classification Tumor necrosis factor superfamily
Havana BLAST/BLAT OTTHUMG00000031194

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