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Recombinant Human Tumor Necrosis Factor α, TNFα (N-6His)

Recombinant Human Tumor Necrosis Factor α, TNFα (N-6His) size: 1 mg 2486

Supplier NOVO
Price 2486
Size 1 mg
Reacts withHuman
Source proteins, Recombinants or rec
DescriptionRecombinant Human Tumor Necrosis Factor alpha is produced by our E, coli expression system and the target gene encoding Gly57-Leu233 is expressed with a 6His tag at the N-terminus
Molecular Weight21, 8 kD
UniProt numberP01375
State of the productFreeze-dried
Shipping conditionsAmbient temperature
Formulation pH 7, 100 mM sodium chloride, 2, 2 um filtered solution of 20 mM PB, Lyophilized from a 0
Storage recommendations -20°, Aliquots of reconstituted samples are stable at less -20°, If kept at at room temperature, If kept at at room temperature, Reconstituted protein solution should be stored at 4-7°, it is stable for 3 weeks, it is stable for 3 weeks, C, C for 2-7 days, C for 3 months, Freeze-dried proteins should be stored frozen at <
Reconstitution Dissolve the lyophilized protein in ddH2O, Do not mix by vortex or pipetting, It is not recommended to reconstitute to a concentration less than 100 &mu, Please aliquot the reconstituted solution to minimize freeze-thaw cycles, Always centrifuge tubes before opening, g/ml
Purity and determined by Polyacrylamide gel electrophoresis, Greater than 95%
Sequence of the amino acidMGSSHHHHHHSSGLVPRGSHMGPQREEFPRDLSLISPLAQAVRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL
Levels of endotoxin11 IEU/ug, LAL test shows less than than 0
Properties Depending on the epitopes used human ELISA kits can be cross reactive to many other species, Mainly analyzed are human serum, Modern , cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies, human cell culture supernatants and biological samples, plasma, primarily , saliva, urine,  , (Homo sapiens, Homo sapiens sapiens), Human proteins, humans , ssp
Grouprecombinants
Gene target (N-6His), TNF&alpha, Tumor Necrosis Factor &alpha
Short name (N-6His), TNF&alpha, Recombinant Tumor Necrosis Factor &alpha
Technique E, coli recombinant proteins are , novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture, supplied as white sterile powder lyopillized, Recombinant, genetic recombinations , in Escherichia coli
SpeciesHumans
Alternative name (N-6His), TNF&alpha, sapiens Tumor Necrosis Factor &alpha, recombinant H
Alternative techniquerec
Identity 11892
Gene TNF
Long gene name tumor necrosis factor
Synonyms gene TNFA
Synonyms gene name member 2) , tumor necrosis factor (TNF superfamily
Synonyms TNFSF2 DIF TNF-alpha
Synonyms name TNF superfamily, member 2
Locus 6p21, 33
Discovery year 1986-01-01
GenBank acession X02910
Entrez gene record 7124
Pubmed identfication 2413547 6392892
Classification Tumor necrosis factor superfamily
Havana BLAST/BLAT OTTHUMG00000031194

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