| Reacts with | Human |
| Source | proteins, Recombinants or rec |
| Description | Activating enzymes will cut off the domain that is biological active to become functional, If the substrate is DNA they are called restriction enzymes, Enzymes are cleaving the substrate |
| Molecular Weight | 17, 5 kD |
| UniProt number | P63146 |
| State of the product | Liquid |
| Shipping conditions | Dry Ice/ice packs |
| Formulation | pH 7, 2 um filtered solution of 10 mM HEPES, 5, Supplied as a 0 |
| Storage recommendations | -20°, Please minimize freeze-thaw cycles, stable for 6 months after receipt, C, Store at < |
| Reconstitution | Dissolve the lyophilized protein in ddH2O, Do not mix by vortex or pipetting, It is not recommended to reconstitute to a concentration less than 100 &mu, Please aliquot the reconstituted solution to minimize freeze-thaw cycles, Always centrifuge tubes before opening, g/ml |
| Purity | and determined by Polyacrylamide gel electrophoresis, Greater than 95% |
| Sequence of the amino acid | GSHMSTPARRRLMRDFKRLQEDPPVGVSGAPSENNIMQWNAVIFGPEGTPFEDGTFKLVIEFSEEYPNKPPTVRFLSKMFHPNVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQSWNDS |
| Levels of endotoxin | 11 IEU/ug, LAL test shows less than than 0 |
| Properties | Depending on the epitopes used human ELISA kits can be cross reactive to many other species, Mainly analyzed are human serum, Modern , cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies, human cell culture supernatants and biological samples, plasma, primarily , saliva, urine,  , (Homo sapiens, Homo sapiens sapiens), Human proteins, humans , ssp |
| Group | recombinants |
| Gene target | HR6B, UBE2B, Ubiquitin-Conjugating E2 B |
| Short name | HR6B, UBE2B, Recombinant Ubiquitin-Conjugating Enzyme E2 B |
| Technique | E, coli recombinant proteins are , enzymes, novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture, supplied as white sterile powder lyopillized, Recombinant, genetic recombinations , in Escherichia coli |
| Species | Humans, Human |
| Alternative name | HR6B, sapiens Ubiquitin-Conjugating Enzyme E2 B, ubiquitin-conjugating enzyme E2B, recombinant H |
| Alternative technique | rec |
| Alternative to gene target | E2-17kDa and HHR6B and HR6B and RAD6B and UBC2, UBE2B and IDBG-44827 and ENSG00000119048 and 7320, UBE2B and IDBG-645802 and ENSBTAG00000010982 and 282107, Ube2b and IDBG-170683 and ENSMUSG00000020390 and 22210, nuclei, this GO :0000209 and protein polyubiquitination and biological process this GO :0000785 and chromatin and cellular component this GO :0000790 and nuclear chromatin and cellular component this GO :0001666 and response to hypoxia and biological process this GO :0001701 and in utero embryonic development and biological process this GO :0001741 and XY body and cellular component this GO :0004842 and ubiquitin-protein transferase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005657 and replication fork and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006281 and DNA repair and biological process this GO :0006301 and postreplication repair and biological process this GO :0006344 and maintenance of chromatin silencing and biological process this GO :0006511 and ubiquitin-dependent protein catabolic process and biological process this GO :0006513 and protein monoubiquitination and biological process this GO :0006974 and cellular response to DNA damage stimulus and biological process this GO :0007283 and spermatogenesis and biological process this GO :0007288 and sperm axoneme assembly and biological process this GO :0008152 and metabolic process and biological process this GO :0009411 and response to UV and biological process this GO :0010845 and positive regulation of reciprocal meiotic recombination and biological process this GO :0016567 and protein ubiquitination and biological process this GO :0016881 and acid-amino acid ligase activity and molecular function this GO :0031056 and regulation of histone modification and biological process this GO :0031625 and ubiquitin protein ligase binding and molecular function this GO :0032869 and cellular response to insulin stimulus and biological process this GO :0033128 and negative regulation of histone phosphorylation and biological process this GO :0033503 and HULC complex and cellular component this GO :0033522 and histone H2A ubiquitination and biological process this GO :0042493 and response to drug and biological process this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0043161 and proteasome-mediated ubiquitin-dependent protein catabolic process and biological process this GO :0043951 and negative regulation of cAMP-mediated signaling and biological process this GO :0050821 and protein stabilization and biological process this GO :0051026 and chiasma assembly and biological process this GO :0051865 and protein autoubiquitination and biological process this GO :0060070 and canonical Wnt signaling pathway and biological process this GO :0070076 and histone lysine demethylation and biological process this GO :0070193 and synaptonemal complex organization and biological process this GO :0070534 and protein K63-linked ubiquitination and biological process this GO :0070936 and protein K48-linked ubiquitination and biological process this GO :0070979 and protein K11-linked ubiquitination and biological process, this GO :0004842: ubiquitin-protein transferase activity, this GO :0004842: ubiquitin-protein transferase activity and also this GO :0005515: protein binding and also this GO :0005524: ATP binding and also this GO :0016881: acid-amino acid ligase activity and also this GO :0031625: ubiquitin protein ligase binding, this GO :0005515: protein binding, this GO :0005524: ATP binding, this GO :0016881: acid-amino acid ligase activity, this GO :0031625: ubiquitin protein ligase binding, ubiquitin protein ligase binding, 512207, ubiquitin-conjugating enzyme E2B |
| Identity | 12473 |
| Gene | UBE2B |
| Long gene name | ubiquitin conjugating enzyme E2 B |
| Synonyms gene name | ubiquitin-conjugating enzyme E2B (RAD6 homolog) ubiquitin-conjugating enzyme E2B |
| Synonyms | UBC2 HHR6B RAD6B |
| Locus | 5q31, 1 |
| Discovery year | 1992-12-02 |
| GenBank acession | M74525 |
| Entrez gene record | 7320 |
| Pubmed identfication | 1559696 |
| RefSeq identity | NM_003337 |
| Classification | Ubiquitin conjugating enzymes E2 |
| Havana BLAST/BLAT | OTTHUMG00000129120 |