Filters

RGS5 Antibody, FITC Conjugated

RGS5 Antibody, FITC Conjugated size: 0.1ml 333

Price 333
Size 0.1ml
Long name FITC Conjugated, RGS5 Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenRGS5
Specificity This antibody may have a minor secondary cross-reactivity to the related proteins RGS3 and RGS4 based on a 67% and 73% non-sequential sequence similarity, respectively, This is a highly specific antibody against RGS5
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen_range80-130/181
Subcellular location Cell membrane, Cytoplasm
SourceKLH conjugated synthetic peptide derived from mouse RGS5
Gene ID number19737
Swiss ProtO08850
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Rat, Mouse
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Binds to G(i)-alpha and G(o)-alpha, but not to G(s)-alpha, Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetRGS5 Conjugated
Short name FITC Conjugated, RGS5 Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, RGS5 (Antibody to)
Alternative techniqueantibodies
Identity 10001
Gene RGS5
Long gene name regulator of G-protein signaling 5
Synonyms gene name regulator of G-protein signalling 5
Locus 1q23, 3
Discovery year 1998-10-12
GenBank acession AF030108
Entrez gene record 8490
Pubmed identfication 9747037
RefSeq identity NM_003617
Classification Regulators of G-protein signaling
Havana BLAST/BLAT OTTHUMG00000034441

Subscribe to our Newsletter