| Long name | RNA polymerase II CTD repeat YSPTSPS (Ser5) Antibody |
| Also known as | RNA polymerase II CTD repeat YSPTSPS (Ser5) Antibody |
| Category | Primary Antibodies |
| Conjugation | Unconjugated |
| Target Antigen | RNA polymerase II CTD repeat YSPTSPS phospho Ser5 |
| Specificity | This is a highly specific antibody against RNA polymerase II CTD repeat YSPTSPS phospho Ser5 |
| Modification(s) | Phosphorylation |
| Modification site(s) | Ser5 |
| Clonality | Polyclonal antibody |
| Clone number | Polyclonal antibody |
| Concentration | 1ug per 1ul |
| Source | KLH conjugated synthetic phosphopeptide derived from human RNA polymerase II CTD repeat YSPTSPS around the phosphorylation site of phospho S5 |
| Gene ID number | 6193 |
| Tested Applications | IF(IHC-P), IHC-P, WB |
| Recommended dilutions | IF(IHC-P)(1:50-200), IHC-P(1:100-500), WB(1:100-1000) |
| Cross reactivity | Mouse, Rat, Human |
| Cross reactive species details | not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the target antigen | A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition, Acts as a RNA-dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, At the start of transcription, During transcription elongation, Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), Forms the polymerase active center together with the second largest subunit, It is composed of mobile elements that move relative to each other, Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs, Pol II is the central component of the basal RNA polymerase II transcription machinery, Pol II moves on the template as the transcript elongates, RPB1 is part of the core element with the central large cleft, a single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II, acting both as a replicate and transcriptase for the viral RNA circular genome, elongation, termination and mRNA processing, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template, which serves as a platform for assembly of factors that regulate transcription initiation, DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates |
| Purification method | This antibody was purified via Protein A |
| Storage conditions | 50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to 1 year, Keep the antibody in aqueous buffered solution containing 1% BSA |
| Synonym names | DNA directed RNA polymerase II A, DNA-directed RNA polymerase II largest subunit, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit, Polr2a, RNA pol II CTD, RNA polymerase II subunit B1, RNA-directed RNA polymerase II subunit RPB1, RPB1, RPB1_HUMAN, p-Rpb1 CTDSer2/Ser5, p-Rpb1 CTD Ser2/Ser5 |
| Properties | C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24° |
| Gene target | RNA polymerase II CTD repeat YSPTSPS (Ser5) |
| Short name | RNA polymerase II CTD repeat YSPTSPS (Ser5) Antibody |
| Technique | antibodies against human proteins, antibodies for, Antibody |
| Alternative name | RNA polymerizing enzyme II CTD repeat YSPTSPS (Ser5) (Antibody to) |
| Alternative technique | antibodies |
| Identity | 2497 |
| Gene | CTD |
| Long gene name | Coats disease |
| Locus | reserved |
| Discovery year | 1994-01-05 |
| Entrez gene record | 1283 |
| Pubmed identfication | 11884883 10484772 |
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