| Long name | Cy3 Conjugated, RNA polymerase II CTD repeat YSPTSPS (Ser5) Antibody |
| Category | Conjugated Primary Antibodies |
| Conjugation | cy3 conjugation kit |
| Host Organism | Rabbit (Oryctolagus cuniculus) |
| Target Antigen | RNA polymerase II CTD repeat YSPTSPS phospho Ser5 |
| Specificity | This is a highly specific antibody against RNA polymerase II CTD repeat YSPTSPS phospho Ser5 |
| Modification | Phosphorylation |
| Modification site | Ser5 |
| Clonality | Polyclonal |
| Clone | Polyclonal antibody |
| Concentration | 1ug per 1ul |
| Source | KLH conjugated synthetic phosphopeptide derived from human RNA polymerase II CTD repeat YSPTSPS around the phosphorylation site of phospho S5 |
| Gene ID number | 6193 |
| Tested applications | IF(IHC-P) |
| Recommended dilutions | IF(IHC-P)(1:50-200) |
| Crossreactivity | Mouse, Rat, Human |
| Crossreactive species details | not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background | A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition, Acts as a RNA-dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, At the start of transcription, During transcription elongation, Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), Forms the polymerase active center together with the second largest subunit, It is composed of mobile elements that move relative to each other, Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs, Pol II is the central component of the basal RNA polymerase II transcription machinery, Pol II moves on the template as the transcript elongates, RPB1 is part of the core element with the central large cleft, a single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II, acting both as a replicate and transcriptase for the viral RNA circular genome, elongation, termination and mRNA processing, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template, which serves as a platform for assembly of factors that regulate transcription initiation, DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates |
| Purification method | This antibody was purified via Protein A |
| Storage conditions | 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission | 512, 550nm/570, 615nm |
| Properties | 1 of the RNA polymerase II CTD repeat YSPTSPS (Ser5) Antibody, Conjugated can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC, Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser, This Bioss Primary Conjugated Antibodies antibody is well suited for fluorescent microscopy, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target | RNA polymerase II CTD repeat YSPTSPS (Ser5) Conjugated |
| Short name | Conjugated, RNA polymerase II CTD repeat YSPTSPS (Ser5) Antibody |
| Technique | antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label | Cy3 |
| Alternative name | cyanine 3 coupled, RNA polymerizing enzyme II CTD repeat YSPTSPS (Ser5) (Antibody to) |
| Alternative technique | antibodies |
| Identity | 2497 |
| Gene | CTD |
| Long gene name | Coats disease |
| Locus | reserved |
| Discovery year | 1994-01-05 |
| Entrez gene record | 1283 |
| Pubmed identfication | 11884883 10484772 |
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