| Long name | FITC Conjugated, RNA polymerase II CTD repeat YSPTSPS (Ser5) Antibody |
| Category | Conjugated Primary Antibodies |
| Conjugation | Anti-FITC Antibody |
| Host Organism | Rabbit (Oryctolagus cuniculus) |
| Target Antigen | RNA polymerase II CTD repeat YSPTSPS phospho Ser5 |
| Specificity | This is a highly specific antibody against RNA polymerase II CTD repeat YSPTSPS phospho Ser5 |
| Modification | Phosphorylation |
| Modification site | Ser5 |
| Clonality | Polyclonal |
| Clone | Polyclonal antibody |
| Concentration | 1ug per 1ul |
| Source | KLH conjugated synthetic phosphopeptide derived from human RNA polymerase II CTD repeat YSPTSPS around the phosphorylation site of phospho S5 |
| Gene ID number | 6193 |
| Tested applications | IF(IHC-P) |
| Recommended dilutions | IF(IHC-P)(1:50-200) |
| Crossreactivity | Mouse, Rat, Human |
| Crossreactive species details | not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background | A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition, Acts as a RNA-dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, At the start of transcription, During transcription elongation, Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), Forms the polymerase active center together with the second largest subunit, It is composed of mobile elements that move relative to each other, Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs, Pol II is the central component of the basal RNA polymerase II transcription machinery, Pol II moves on the template as the transcript elongates, RPB1 is part of the core element with the central large cleft, a single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II, acting both as a replicate and transcriptase for the viral RNA circular genome, elongation, termination and mRNA processing, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template, which serves as a platform for assembly of factors that regulate transcription initiation, DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates |
| Purification method | This antibody was purified via Protein A |
| Storage conditions | 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission | 494nm/518nm |
| Properties | Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target | RNA polymerase II CTD repeat YSPTSPS (Ser5) Conjugated |
| Short name | FITC Conjugated, RNA polymerase II CTD repeat YSPTSPS (Ser5) Antibody |
| Technique | Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label | FITC |
| Alternative name | fluorecein coupled, RNA polymerizing enzyme II CTD repeat YSPTSPS (Ser5) (Antibody to) |
| Alternative technique | antibodies |
| Identity | 2497 |
| Gene | CTD |
| Long gene name | Coats disease |
| Locus | reserved |
| Discovery year | 1994-01-05 |
| Entrez gene record | 1283 |
| Pubmed identfication | 11884883 10484772 |
Technique
Supplier
Species
Label
Iso Type
Host
Tissue