Name : RNF158 Antibody, Biotin Conjugated
Supplier : BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price :333
SKU : GEN5868145743
| Long name | Biotin Conjugated, RNF158 Polyclonal Antibody |
| Category | Conjugated Primary Antibodies |
| Conjugation | Biotinylated |
| Host Organism | Rabbit (Oryctolagus cuniculus) |
| Target Antigen | RNF158 |
| Specificity | This is a highly specific antibody against RNF158 |
| Modification | Unmodified |
| Modification site | None |
| Clonality | Polyclonal |
| Clone | Polyclonal antibody |
| Concentration | 1ug per 1ul |
| Source | KLH conjugated synthetic peptide derived from human RNF158 |
| Gene ID number | 153769 |
| Tested applications | IHC-P, WB |
| Recommended dilutions | IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity | Mouse, Rat, Human |
| Crossreactive species details | not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background | Due to alternative splicing events, Proteins that contain this conserved domain are generally involved in the ubiquitination pathway of protein degradation, SH3RF2 (SH3 domain containing ring finger 2), SH3RF2 binds an E2 ubiquitin-conjugating enzyme, SH3RF2 is expressed as two different isoforms, This suggests that SH3RF2 functions as an E3 ubiquitin-protein ligase that accepts a ubiquitin residue from an E2 ubiquitin-conjugating enzyme and immediately transfers that residue to a protein that is targeted for degradation, Via its RING-type zinc finger domain, also known as RNF158, is a 729 amino acid protein with one RING-type zinc finger domain and three SH3 domains, The RING-type zinc finger motif is present in a number of viral and eukaryotic proteins and is made of a conserved cysteine-rich domain that is able to bind two zinc atoms |
| Purification method | This antibody was purified via Protein A |
| Storage conditions | 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission | N/A |
| Synonyms | FLJ23654 RNF158 Hepp1 POSHER |
| Properties | Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target | RNF158 Conjugated |
| Short name | Conjugated, RNF158 Antibody |
| Technique | antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label | Biotin |
| Alternative name | biotinilated coupled, RNF158 (Antibody to) |
| Alternative technique | antibodies |
| Identity | 26299 |
| Gene | SH3RF2 |
| Long gene name | SH3 domain containing ring finger 2 |
| Synonyms gene | PPP1R39 |
| Synonyms gene name | protein phosphatase 1, regulatory subunit 39 |
| Synonyms name | heart protein phosphatase 1-binding protein POSH-eliminating RING protein |
| Locus | 5q32 |
| Discovery year | 2004-03-05 |
| GenBank acession | AL833297 |
| Entrez gene record | 153769 |
| Pubmed identfication | 22128169 |
| RefSeq identity | NM_152550 |
| Classification | Ring finger proteins Protein phosphatase 1 regulatory subunits |
| Havana BLAST/BLAT | OTTHUMG00000129685 |