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RNF27 Antibody, Biotin Conjugated

RNF27 Antibody, Biotin Conjugated size: 0.1ml 333

Price 333
Size 0.1ml
Long name Biotin Conjugated, RNF27 Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationBiotinylated
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenRNF27
SpecificityThis is a highly specific antibody against RNF27
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic peptide derived from human TRIM8/RNF27
Gene ID number81603
Tested applications IHC-P, WB
Recommended dilutions IHC-P(1:100-500), WB(1:100-1000)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background As a widely expressed homodimer, TRIM8 (tripartite motif containing 8), TRIM8 is expressed in lung, TRIM8 localizes to nuclear bodies and contains two B box-type zinc fingers and one RING-type zinc finger, The gene encoding TRIM8 maps to human chromosome 10q24, a B-box type zinc finger, also known as GERP (glioblastoma-expressed RING finger protein) or RNF27 (RING finger protein 27), brain and skeletal muscle, heart, is a 551 amino acid protein that is thought to function as an E3 ubiquitin-protein ligase that promotes SOCS-1 proteasomal degradation, leukocytes and liver, one RING finger and three zinc-binding domains, placenta, with low levels detected in intestine, 32, The tripartite motif (TRIM) family of proteins are characterized by a conserved TRIM domain that includes a coiled-coil region
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation EmissionN/A
Synonyms GERP
Properties Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetRNF27 Conjugated
Short name Conjugated, RNF27 Antibody
Technique antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelBiotin
Alternative name biotinilated coupled, RNF27 (Antibody to)
Alternative techniqueantibodies
Identity 15579
Gene TRIM8
Long gene name tripartite motif containing 8
Synonyms gene RNF27
Synonyms gene name ring finger protein 27 tripartite motif-containing 8
Synonyms name glioblastoma expressed ring finger protein
Locus 10q24, 32
Discovery year 2001-04-26
GenBank acession AF281046
Entrez gene record 81603
Pubmed identfication 11118312 12163497
RefSeq identity NM_030912
Classification Ring finger proteins Tripartite motif containing
Havana BLAST/BLAT OTTHUMG00000018964

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