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RNF74 Antibody, Cy5.5 Conjugated

RNF74 Antibody, Cy5.5 Conjugated size: 0.1ml 350

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 350
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name Cy5, 5 Conjugated, RNF74 Polyclonal Antibody
CategoryConjugated Primary Antibodies
Conjugation Cy5, 5, 5, Cy5
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenRNF74
SpecificityThis is a highly specific antibody against RNF74
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen_range385-430/1043
Subcellular locationNucleus
SourceKLH conjugated synthetic peptide derived from human RAG1/RNF74
Gene ID number5896
Swiss ProtP15918
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background 5'-phosphorylated ends, DNA cleavage occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, Histone H3 monoubiquitination is required for the joining step of V(D)J recombination, In addition to its endonuclease activity, In the RAG complex, RAG1 also acts as a E3 ubiquitin-protein ligase that mediates monoubiquitination of histone H3, RAG1 mediates the DNA-binding to the conserved recombination signal sequences (RSS) and catalyzes the DNA cleavage activities by introducing a double-strand break between the RSS and the adjacent coding segment, RAG2 is not a catalytic component but is required for all known catalytic activities, The RAG complex also plays a role in pre-B cell allelic exclusion, The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps, The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes, and J (joining) gene segments, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, in some cases D (diversity), preventing accessibility to the RAG complex and recombination of the second allele, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, Catalytic component of the RAG complex
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission675nm/694nm
Synonyms RNF74 MGC43321
Properties Cy5 antibodies are excited by the 650-nanometer wave of an argon laser and detected at a 670-nanometer captor, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
ConjugatedThese antibodies are excite for emission at 678nm and detected at a 694 , nm wavelengths
Gene targetRNF74 Conjugated
Short name Conjugated, RNF74 Antibody
Technique antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
Label5, Cy5
Alternative name cyanine 5, 5 coupled, RNF74 (Antibody to)
Alternative techniqueantibodies
Identity 9831
Gene RAG1
Long gene name recombination activating 1
Synonyms gene name recombination activating gene 1
Synonyms name recombination activating protein 1 RING finger protein 74 V(D)J recombination-activating protein 1
Locus 11p12
Discovery year 1990-06-18
GenBank acession M29474
Entrez gene record 5896
Pubmed identfication 1612612 1283330
RefSeq identity NM_000448
Classification Ring finger proteins
Havana BLAST/BLAT OTTHUMG00000166392
Locus Specific Databases RAG1base: Mutation registry for autosomal recessive RAG1 deficiency LRG_98

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