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RPA2(Thr21) Antibody, Biotin Conjugated

RPA2(Thr21) Antibody, Biotin Conjugated size: 0.1ml 350

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 350
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name Biotin Conjugated, RPA2(Thr21) Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationBiotinylated
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenRPA2Thr21
SpecificityThis is a highly specific antibody against RPA2Thr21
ModificationPhosphorylation
Modification siteThr21
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen_range5-40/270
Subcellular locationNucleus
SourceKLH conjugated synthetic phosphopeptide derived from human RPA2 around the phosphorylation site of Thr21
Gene ID number6118
Swiss ProtP15927
Tested applications IHC-P, WB
Recommended dilutions IHC-P(1:100-500), WB(1:100-1000)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Also recruits SMARCAL1/HARP, Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair, In the cellular response to DNA damage, It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage, It prevents their reannealing and in parallel, May also play a role in telomere maintenance, Plays also a role in base excision repair (BER) probably through interaction with UNG, Thereby, Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control, Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response, binds and stabilizes single-stranded DNA intermediates, it plays an essential role both in DNA replication and the cellular response to DNA damage, recruits and activates different proteins and complexes involved in DNA metabolism, that form during DNA replication or upon DNA stress, the RPA complex controls DNA repair and DNA damage checkpoint activation, to sites of DNA damage, which is involved in replication fork restart, As part of the heterotrimeric replication protein A complex (RPA/RP-A)
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation EmissionN/A
Properties Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetRPA2(Thr21) Conjugated
Short name Conjugated, RPA2(Thr21) Antibody
Technique antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelBiotin
Alternative name biotinilated coupled, RPA2(Thr21) (Antibody to)
Alternative techniqueantibodies
Identity 10290
Gene RPA2
Long gene name replication protein A2
Synonyms gene name 32kDa , replication protein A2 (32kD) replication protein A2
Locus 1p35, 3
Discovery year 1993-12-14
GenBank acession BC021257
Entrez gene record 6118
Pubmed identfication 8454588
RefSeq identity NM_002946
Classification Nucleotide excision repair
Havana BLAST/BLAT OTTHUMG00000003915

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