| Antigen detectable with this ELISA kit | S100 Calcium Binding Protein A7 (S100A7) |
| Species reactivity | Homo sapiens (Human) |
| Range of detection | 1000pg/mL, 125pg/mL, 2000pg/mL, 250pg/mL, 4000pg/mL, 500pg/mL, 125-8000pg/mL The standard curve concentrations used for the ELISA&rsquo, s were 8000pg/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 48pg/mL |
| Application | ELISA |
| Sample types tested for this ELISA kit | cell lysates and other biological fluids, plasma, tissue homogenates, Serum |
| Time for performing t hourse test | 4, 5 hoursours |
| Test principle | 10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to S100 Calcium Binding Protein A7 (S100A7), The concentration of S100 Calcium Binding Protein A7 (S100A7) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to S100 Calcium Binding Protein A7 (S100A7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain S100 Calcium Binding Protein A7 (S100A7), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling | Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory | Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties | E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test | ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target | S100 Calcium Binding Protein A7 (S100A7) |
| Short name | S100 Calcium Binding Protein A7 (S100A7) ELISA kit |
| Technique | &hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host | antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name | S100 Calcium Binding Protein A7 (S100 calcium binding protein A7) Enzyme-linked immunosorbent assay reagent |
| Alternative technique | elisas |
| Alternative to gene target | PSOR1 and S100A7c, RAGE receptor binding, S100A7 and IDBG-102676 and ENSG00000143556 and 6278, nuclei, this GO :0000302 and response to reactive oxygen species and biological process this GO :0001525 and angiogenesis and biological process this GO :0005509 and calcium ion binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005783 and endoplasmic reticulum and cellular component this GO :0005829 and cytosol and cellular component this GO :0005925 and focal adhesion and cellular component this GO :0008270 and zinc ion binding and molecular function this GO :0008544 and epidermis development and biological process this GO :0010820 and positive regulation of T cell chemotaxis and biological process this GO :0030216 and keratinocyte differentiation and biological process this GO :0032496 and response to lipopolysaccharide and biological process this GO :0045087 and innate immune response and biological process this GO :0050786 and RAGE receptor binding and molecular function this GO :0050829 and defense response to Gram-negative bacterium and biological process this GO :0051238 and sequestering of metal ion and biological process this GO :0070062 and extracellular vesicular exosome and cellular component this GO :0070374 and positive regulation of ERK1 and ERK2 cascade and biological process this GO :0071624 and positive regulation of granulocyte chemotaxis and biological process this GO :0090026 and positive regulation of monocyte chemotaxis and biological process, this GO :0005509: calcium ion binding, this GO :0005509: calcium ion binding and also this GO :0005515: protein binding and also this GO :0008270: zinc ion binding and also this GO :0050786: RAGE receptor binding, this GO :0005515: protein binding, this GO :0008270: zinc ion binding, this GO :0050786: RAGE receptor binding, S100 calcium binding protein A7 |
| Identity | 10497 |
| Gene | S100A7 |
| Long gene name | S100 calcium binding protein A7 |
| Synonyms gene | PSOR1 |
| Synonyms gene name | S100 calcium-binding protein A7 (psoriasin 1) S100 calcium binding protein A7 (psoriasin 1) |
| Synonyms | S100A7c |
| Locus | 1q21, 3 |
| Discovery year | 1992-10-08 |
| GenBank acession | BC034687 |
| Entrez gene record | 6278 |
| Pubmed identfication | 1940442 |
| RefSeq identity | NM_002963 |
| Classification | S100 calcium binding proteins EF-hand domain containing |
| Havana BLAST/BLAT | OTTHUMG00000013123 |