| Long name | FITC Conjugated, S100A8 Polyclonal Antibody |
| Category | Conjugated Primary Antibodies |
| Conjugation | Anti-FITC Antibody |
| Host Organism | Rabbit (Oryctolagus cuniculus) |
| Target Antigen | S100A8 |
| Specificity | This is a highly specific antibody against S100A8 |
| Modification | Unmodified |
| Modification site | None |
| Clonality | Polyclonal |
| Clone | Polyclonal antibody |
| Concentration | 1ug per 1ul |
| Immunogen_range | 20-60/89 |
| Subcellular location | Cell membrane, Secreted, Cytoplasm |
| Source | KLH conjugated synthetic peptide derived from mouse S100-A8 |
| Gene ID number | 6279 |
| Swiss Prot | P05109 |
| Tested applications | IF(IHC-P) |
| Recommended dilutions | IF(IHC-P)(1:50-200) |
| Crossreactivity | Rat, Mouse |
| Crossreactive species details | not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background | Activates NADPH-oxidase by facilitating the enzyme complex assembly at the cell membrane, Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER), Binding to TLR4 and AGER activates the MAP-kinase and NF-kappa-B signaling pathways resulting in the amplification of the proinflammatory cascade, Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3, Can regulate neutrophil number and apoptosis by an anti-apoptotic effect, Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn(2+) which is essential for microbial growth, It can induce neutrophil chemotaxis and adhesion, Its proinflammatory activity includes recruitment of leukocytes, Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions, The extracellular functions involve proinfammatory, The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, an essential cofactor, and regulation of leukocyte adhesion and migration, antimicrobial, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase, oxidant-scavenging and apoptosis-inducing activities, promotion of cytokine and chemokine production, regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX, transferring arachidonic acid, S100A8 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response |
| Purification method | This antibody was purified via Protein A |
| Storage conditions | 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission | 494nm/518nm |
| Synonyms | P8 MRP8 60B8AG CGLA |
| Properties | Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target | S100A8 Conjugated |
| Short name | FITC Conjugated, S100A8 Antibody |
| Technique | Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label | FITC |
| Alternative name | fluorecein coupled, S100 calcium binding protein A8 (Antibody to) |
| Alternative technique | antibodies |
| Alternative to gene target | 60B8AG and CAGA and CFAG and CGLA and CP-10 and L1Ag and MA387 and MIF and MRP8 and NIF and P8, RAGE receptor binding, S100A8 and IDBG-102654 and ENSG00000143546 and 6279, S100A8 and IDBG-632868 and ENSBTAG00000012640 and 616818, S100a8 and IDBG-168077 and ENSMUSG00000056054 and 20201, nuclei, this GO :0001816 and cytokine production and biological process this GO :0002523 and leukocyte migration involved in inflammatory response and biological process this GO :0002526 and acute inflammatory response and biological process this GO :0002544 and chronic inflammatory response and biological process this GO :0005509 and calcium ion binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005615 and extracellular space and cellular component this GO :0005634 and nucleus and cellular component this GO :0005829 and cytosol and cellular component this GO :0005856 and cytoskeleton and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006914 and autophagy and biological process this GO :0006919 and activation of cysteine-type endopeptidase activity involved in apoptotic process and biological process this GO :0006954 and inflammatory response and biological process this GO :0008017 and microtubule binding and molecular function this GO :0008270 and zinc ion binding and molecular function this GO :0010043 and response to zinc ion and biological process this GO :0030307 and positive regulation of cell growth and biological process this GO :0030593 and neutrophil chemotaxis and biological process this GO :0032119 and sequestering of zinc ion and biological process this GO :0032496 and response to lipopolysaccharide and biological process this GO :0032602 and chemokine production and biological process this GO :0035662 and Toll-like receptor 4 binding and molecular function this GO :0042060 and wound healing and biological process this GO :0042742 and defense response to bacterium and biological process this GO :0045087 and innate immune response and biological process this GO :0045471 and response to ethanol and biological process this GO :0050544 and arachidonic acid binding and molecular function this GO :0050729 and positive regulation of inflammatory response and biological process this GO :0050786 and RAGE receptor binding and molecular function this GO :0050832 and defense response to fungus and biological process this GO :0051092 and positive regulation of NF-kappaB transcription factor activity and biological process this GO :0051493 and regulation of cytoskeleton organization and biological process this GO :0070062 and extracellular vesicular exosome and cellular component this GO :0070488 and neutrophil aggregation and biological process this GO :2001244 and positive regulation of intrinsic apoptotic signaling pathway and biological process, this GO :0005509: calcium ion binding, this GO :0005509: calcium ion binding and also this GO :0005515: protein binding and also this GO :0008017: microtubule binding and also this GO :0008270: zinc ion binding and also this GO :0035662: Toll-like receptor 4 binding and also this GO :0050544: arachidonic acid binding and also this GO :0050786: RAGE receptor binding, this GO :0005515: protein binding, this GO :0008017: microtubule binding, this GO :0008270: zinc ion binding, this GO :0035662: Toll-like receptor 4 binding, this GO :0050544: arachidonic acid binding, this GO :0050786: RAGE receptor binding, S100 calcium binding protein A8 |
| Identity | 10498 |
| Gene | S100A8 |
| Long gene name | S100 calcium binding protein A8 |
| Synonyms gene | CAGA CFAG |
| Synonyms gene name | S100 calcium-binding protein A8 (calgranulin A) S100 calcium binding protein A8 (calgranulin A) |
| Locus | 1q21, 3 |
| Discovery year | 1989-05-19 |
| GenBank acession | BC005928 |
| Entrez gene record | 6279 |
| RefSeq identity | NM_002964 |
| Classification | S100 calcium binding proteins EF-hand domain containing |
| Havana BLAST/BLAT | OTTHUMG00000013124 |
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