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SAP155 Antibody, FITC Conjugated

SAP155 Antibody, FITC Conjugated size: 0.1ml 333

Price 333
Size 0.1ml
Long name FITC Conjugated, SAP155 Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenSAP155
SpecificityThis is a highly specific antibody against SAP155
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic peptide derived from human SAP155/SF3B1
Gene ID number23451
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Concomitant with splicing catalysis, Due to alternative splicing events, Localized to nuclear speckles and also to the cytoplasm during mitosis, SAP 130, SAP 145 and SAP 155, SAP 155 is a subunit of the SF3B splicing factor, SAP 155 is phosphorylated at its N-terminal Thr-Pro dipeptide motifs by Dyrk1A and cyclin E/Cdk2, SF3B contains the spliceosomal proteins SAP 49, The SF3B splicing factor is a U2 snRNP-associated protein complex essential for spliceosome assembly, This modification of SAP 155 is vital for a functional spliceosome as it is an essential event in the basic splicing reaction, various SAP 155 isoforms are produced, 304 amino acid member of the SF3B1 family and contains eleven HEAT repeats, SAP 155 is a 1
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Synonyms SAP155 SF3b155 PRPF10 Prp10 Hsh155
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetSAP155 Conjugated
Short name FITC Conjugated, SAP155 Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, SAP155 (Antibody to)
Alternative techniqueantibodies
Identity 10768
Gene SF3B1
Long gene name splicing factor 3b subunit 1
Synonyms gene name 155kD splicing factor 3b, 155kDa , splicing factor 3b, subunit 1, subunit 1
Locus 2q33, 1
Discovery year 2000-02-29
GenBank acession AF054284
Entrez gene record 23451
Pubmed identfication 9585501
Havana BLAST/BLAT OTTHUMG00000154447
Locus Specific Databases LRG_624

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