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SHC2/Sck Antibody, FITC Conjugated

SHC2/Sck Antibody, FITC Conjugated size: 0.1ml 333

Price 333
Size 0.1ml
Long name FITC Conjugated, SHC2/Sck Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenSHC2/Sck
SpecificityThis is a highly specific antibody against SHC2/Sck
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic peptide derived from human SHC2/Sck
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Human Sck transcripts are present at high levels in liver, In vascular endothelial cells, Sck participates in VEGF-induced signal transduction (6), Shc-like protein (Sck) is a neuronal adaptor protein that contains an N-terminal PTB (phosphotyrosine binding) domain, Treatment of human umbilical vein endothelial (HUVEC) cells with VEGF induces recruitment of Sck to tyrosine-1175 of the kinase insert domain-containing receptor (KDR) and enhances Sck tyrosine phosphorylation (7, a collagen homology (CH) domain, and a conserved C-terminal SH2 domain (2, pancreas, prostate and ovary (4, 3), 5), 8), Src homology 2 (SH2) domains bind specifically to tyrosine-phosphorylated proteins that temporally participate in signal transduction events (1)
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Synonyms SLI SCK SHCB
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetSHC2/Sck Conjugated
Short name FITC Conjugated, SHC2/Sck Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, SHC2/Sck (Antibody to)
Alternative techniqueantibodies
Identity 29869
Gene SHC2
Long gene name SHC adaptor protein 2
Synonyms gene name SHC (Src homology 2 domain containing) transforming protein 2
Synonyms name neuronal Shc adaptor homolog
Locus 19p13, 3
Discovery year 2004-03-18
GenBank acession AB001451
Entrez gene record 25759
Pubmed identfication 7527937 9507002
Classification SH2 domain containing
Havana BLAST/BLAT OTTHUMG00000180545

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