| Long name | ALEXA FLUOR 555 Conjugated, SLIT2/Slil3 Polyclonal Antibody |
| Also known as | Anti-SLIT2/Slil3 PAb ALEXA FLUOR 555 |
| Category | Conjugated Primary Antibodies |
| Conjugated with | 555, ALEXA FLUOR® |
| Host Organism | Rabbit (Oryctolagus cuniculus) |
| Target Antigen | SLIT2/Slil3 |
| Specificity | This is a highly specific antibody against SLIT2/Slil3 |
| Modification | Unmodified |
| Modification Site | None |
| Clonality | Polyclonal |
| Clone | Polyclonal antibody |
| Concentration | 1ug per 1ul |
| Immunogen range | 470-520/1529 |
| Subcellular location | Secreted |
| Source | This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human Slit2 |
| Gene ID Number | 9353 |
| Swiss Prot | O94813 |
| Tested applications | IF(IHC-P) |
| Recommended dilutions | IF(IHC-P)(1:50-200) |
| Crossreactivity | Mouse, Rat, Human |
| Cross-reactive species details | not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the antigen | During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions, In spinal chord development may play a role in guiding commissural axons once they reached the floor plate by modulating the response to netrin, In the developing visual system appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, In vitro, In vitro, In vitro, In vitro, May be implicated in spinal chord midline post-crossing axon repulsion, SLIT1 and SLIT2 seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb, Seems to be involved in regulating leukocyte migration, Seems to play a role in branching and arborization of CNS sensory axons, Slit homolog 2 protein N-product, and after passage through, and function appears to be mediated by interaction with roundabout homolog receptors, and in neuronal cell migration, but not Slit homolog 2 protein C-product, collapses and repels retinal ganglion cell growth cones, induces OB growth cones collapse and induces branching of DRG axons, only commissural axons that crossed the midline responded to SLIT2, repels olfactory bulb (OB) but not dorsal root ganglia (DRG) axons, silences the attractive effect of NTN1 but not its growth-stimulatory effect and silencing requires the formation of a ROBO1-DCC complex, the optic chiasm, Thought to act as molecular guidance cue in cellular migration |
| Purification | Purified by Protein A |
| Storage conditions | 50% glycerol and 0, Keep refrigerated at 2 to 8 degrees Celcius for up to one year, 09% sodium azide, Store this antibody in aqueous buffered solution containing 1% BSA |
| Excitation emission | 553nm/568nm |
| Synonyms | Slit-2 |
| Properties | ALEXA FLUOR they should be stored frozen at - 24°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies, Very high photo stable ALEXA conjugate, C, C for long term storage and for short term at + 5°, For facs or microscopy Alexa 1 conjugate |
| Conjugation | ALEXA FLUOR 555, Alexa Fluor |
| Gene target | SLIT2/Slil3 |
| Short name | SLIT2/Slil3 Antibody |
| Technique | antibodies against human proteins, antibodies for, Antibody |
| Label | ALEXA FLUOR 555 |
| Alternative name | ALEXA FLUOR 555, slit homolog 2 (Drosophila)/Slil3 (Antibody to) |
| Alternative technique | antibodies |
| Alternative to gene target | Extracellular, Roundabout binding, SLIL3 and Slit-2, SLIT2 and IDBG-11255 and ENSG00000145147 and 9353, SLIT2 and IDBG-641256 and ENSBTAG00000005108 and 534164, Slit2 and IDBG-162862 and ENSMUSG00000031558 and 20563, this GO :0001656 and metanephros development and biological process this GO :0001657 and ureteric bud development and biological process this GO :0001701 and in utero embryonic development and biological process this GO :0001933 and negative regulation of protein phosphorylation and biological process this GO :0002042 and cell migration involved in sprouting angiogenesis and biological process this GO :0002689 and negative regulation of leukocyte chemotaxis and biological process this GO :0005095 and GTPase inhibitor activity and molecular function this GO :0005509 and calcium ion binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005615 and extracellular space and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0007409 and axonogenesis and biological process this GO :0007411 and axon guidance and biological process this GO :0008045 and motor neuron axon guidance and biological process this GO :0008201 and heparin binding and molecular function this GO :0008285 and negative regulation of cell proliferation and biological process this GO :0009986 and cell surface and cellular component this GO :0010593 and negative regulation of lamellipodium assembly and biological process this GO :0010596 and negative regulation of endothelial cell migration and biological process this GO :0010629 and negative regulation of gene expression and biological process this GO :0014912 and negative regulation of smooth muscle cell migration and biological process this GO :0016020 and membrane and cellular component this GO :0016337 and single organismal cell-cell adhesion and biological process this GO :0021772 and olfactory bulb development and biological process this GO :0021836 and chemorepulsion involved in postnatal olfactory bulb interneuron migration and biological process this GO :0021972 and corticospinal neuron axon guidance through spinal cord and biological process this GO :0022029 and telencephalon cell migration and biological process this GO :0030308 and negative regulation of cell growth and biological process this GO :0030336 and negative regulation of cell migration and biological process this GO :0030517 and negative regulation of axon extension and biological process this GO :0030837 and negative regulation of actin filament polymerization and biological process this GO :0031290 and retinal ganglion cell axon guidance and biological process this GO :0032870 and cellular response to hormone stimulus and biological process this GO :0033563 and dorsal/ventral axon guidance and biological process this GO :0035385 and Roundabout signaling pathway and biological process this GO :0042802 and identical protein binding and molecular function this GO :0042803 and protein homodimerization activity and molecular function this GO :0043065 and positive regulation of apoptotic process and biological process this GO :0043086 and negative regulation of catalytic activity and biological process this GO :0043116 and negative regulation of vascular permeability and biological process this GO :0043237 and laminin-1 binding and molecular function this GO :0043394 and proteoglycan binding and molecular function this GO :0045499 and chemorepellent activity and molecular function this GO :0048495 and Roundabout binding and molecular function this GO :0048754 and branching morphogenesis of an epithelial tube and biological process this GO :0048812 and neuron projection morphogenesis and biological process this GO :0048846 and axon extension involved in axon guidance and biological process this GO :0050772 and positive regulation of axonogenesis and biological process this GO :0050919 and negative chemotaxis and biological process this GO :0050929 and induction of negative chemotaxis and biological process this GO :0051058 and negative regulation of small GTPase mediated signal transduction and biological process this GO :0051414 and response to cortisol and biological process this GO :0060603 and mammary gland duct morphogenesis and biological process this GO :0060763 and mammary duct terminal end bud growth and biological process this GO :0061364 and apoptotic process involved in luteolysis and biological process this GO :0070062 and extracellular vesicular exosome and cellular component this GO :0070100 and negative regulation of chemokine-mediated signaling pathway and biological process this GO :0071504 and cellular response to heparin and biological process this GO :0071672 and negative regulation of smooth muscle cell chemotaxis and biological process this GO :0071676 and negative regulation of mononuclear cell migration and biological process this GO :0090024 and negative regulation of neutrophil chemotaxis and biological process this GO :0090027 and negative regulation of monocyte chemotaxis and biological process this GO :0090260 and negative regulation of retinal ganglion cell axon guidance and biological process this GO :0090288 and negative regulation of cellular response to growth factor stimulus and biological process, this GO :0005095: GTPase inhibitor activity, this GO :0005095: GTPase inhibitor activity and also this GO :0005509: calcium ion binding and also this GO :0005515: protein binding and also this GO :0008201: heparin binding and also this GO :0042802: identical protein binding and also this GO :0042803: protein homodimerization activity and also this GO :0043237: laminin-1 binding and also this GO :0043394: proteoglycan binding and also this GO :0045499: chemorepellent activity and also this GO :0048495: Roundabout binding, this GO :0005509: calcium ion binding, this GO :0005515: protein binding, this GO :0008201: heparin binding, this GO :0042802: identical protein binding, this GO :0042803: protein homodimerization activity, this GO :0043237: laminin-1 binding, this GO :0043394: proteoglycan binding, this GO :0045499: chemorepellent activity, this GO :0048495: Roundabout binding, slit homolog 2 (Drosophila) |
| Identity | 11086 |
| Gene | SLIT2 |
| Long gene name | slit guidance ligand 2 |
| Synonyms gene | SLIL3 |
| Synonyms gene name | slit (Drosophila) homolog 2 slit homolog 2 (Drosophila) |
| Locus | 4p15, 31 |
| Discovery year | 1999-06-11 |
| GenBank acession | AF055585 |
| Entrez gene record | 9353 |
| Pubmed identfication | 9813312 18269211 |
| Havana BLAST/BLAT | OTTHUMG00000128551 |
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