| Long name | SMAD2(Ser465) Primary Polyclonal Antibody |
| Also known as | SMAD2(Ser465) PAb |
| Category | Primary Antibodies |
| Conjugation | Unconjugated |
| Target Antigen | SMAD2 Ser465 |
| Specificity | This is a highly specific antibody against SMAD2 Ser465 |
| Modification(s) | Phosphorylation |
| Modification site(s) | Ser465 |
| Clonality | Polyclonal antibody |
| Clone number | Polyclonal antibody |
| Concentration | 1ug per 1ul |
| Immunogen range | 457-467/467 |
| Subcellular location | Cytoplasm |
| Source | KLH conjugated synthetic phosphopeptide derived from human SMAD2 around the phosphorylation site of Ser465 |
| Gene ID number | 4087 |
| Swiss Prot | Q15796 |
| Tested Applications | IF(IHC-P), IHC-P, WB |
| Recommended dilutions | IF(IHC-P)(1:50-200), IHC-P(1:100-500), WB(1:100-1000) |
| Cross reactivity | Mouse, Rat, Human |
| Cross reactive species details | not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the target antigen | Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, May act as a tumor suppressor in colorectal carcinoma, Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator, activates transcription, on formation of the SMAD2/SMAD4 complex, Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases |
| Purification method | This antibody was purified via Protein A |
| Storage conditions | 50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to 1 year, Keep the antibody in aqueous buffered solution containing 1% BSA |
| Synonym names | JV18-1, MAD homolog 2, MADH2, MADR2, Mad-related protein 2, Mothers against DPP homolog 2, Mothers against decapentaplegic homolog 2, SMAD 2, SMAD family member 2, Smad2, hMAD-2, hSMAD2, JV18 |
| Properties | C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24° |
| Gene target | SMAD2(Ser465) |
| Short name | SMAD2(Ser465) Antibody |
| Technique | antibodies against human proteins, antibodies for, Antibody |
| Alternative name | SMAD family member 2(Ser465) (Antibody to) |
| Alternative technique | antibodies |
| Alternative to gene target | DNA-templated and biological process this GO :0006355 and regulation of transcription, DNA-templated and biological process this GO :0006367 and transcription initiation from RNA polymerase II promoter and biological process this GO :0006468 and protein phosphorylation and biological process this GO :0007179 and transforming growth factor beta receptor signaling pathway and biological process this GO :0007182 and common-partner SMAD protein phosphorylation and biological process this GO :0007183 and SMAD protein complex assembly and biological process this GO :0007352 and zy this GO tic specification of dorsal/ventral axis and biological process this GO :0007369 and gastrulation and biological process this GO :0007389 and pattern specification process and biological process this GO :0007492 and endoderm development and biological process this GO :0007507 and heart development and biological process this GO :0008134 and transcription factor binding and molecular function this GO :0008285 and negative regulation of cell proliferation and biological process this GO :0009749 and response to glucose and biological process this GO :0009791 and post-embryonic development and biological process this GO :0009880 and embryonic pattern specification and biological process this GO :0009952 and anterior/posterior pattern specification and biological process this GO :0010467 and gene expression and biological process this GO :0010718 and positive regulation of epithelial to mesenchymal transition and biological process this GO :0017015 and regulation of transforming growth factor beta receptor signaling pathway and biological process this GO :0019902 and phosphatase binding and molecular function this GO :0023019 and signal transduction involved in regulation of gene expression and biological process this GO :0030073 and insulin secretion and biological process this GO :0030324 and lung development and biological process this GO :0030512 and negative regulation of transforming growth factor beta receptor signaling pathway and biological process this GO :0030513 and positive regulation of BMP signaling pathway and biological process this GO :0030618 and transforming growth factor beta receptor, DNA-templated and biological process this GO :0045893 and positive regulation of transcription, DNA-templated and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0046332 and SMAD binding and molecular function this GO :0046872 and metal ion binding and molecular function this GO :0048340 and paraxial mesoderm morphogenesis and biological process this GO :0048589 and developmental growth and biological process this GO :0048617 and embryonic foregut morphogenesis and biological process this GO :0048701 and embryonic cranial skeleton morphogenesis and biological process this GO :0051098 and regulation of binding and biological process this GO :0060021 and palate development and biological process this GO :0060039 and pericardium development and biological process this GO :0070410 and co-SMAD binding and molecular function this GO :0070411 and I-SMAD binding and molecular function this GO :0070412 and R-SMAD binding and molecular function this GO :0070723 and response to cholesterol and biological process this GO :0071141 and SMAD protein complex and cellular component this GO :0071144 and SMAD2-SMAD3 protein complex and cellular component this GO :1900224 and positive regulation of nodal signaling pathway involved in determination of lateral mesoderm left/right asymmetry and biological process, SMAD2 and IDBG-3127 and ENSG00000175387 and 4087, SMAD2 and IDBG-644642 and ENSBTAG00000039916 and 515125, Smad2 and IDBG-159056 and ENSMUSG00000024563 and 17126, hMAD-2 and hSMAD2 and JV18 and JV18-1 and MADH2 and MADR2, nuclei, pathway-specific cytoplasmic mediator activity, pathway-specific cytoplasmic mediator activity and also this GO :0031625: ubiquitin protein ligase binding and also this GO :0033613: activating transcription factor binding and also this GO :0034713: type I transforming growth factor beta receptor binding and also this GO :0035326: enhancer binding and also this GO :0046332: SMAD binding and also this GO :0046872: metal ion binding and also this GO :0070410: co-SMAD binding and also this GO :0070411: I-SMAD binding and also this GO :0070412: R-SMAD binding, pathway-specific cytoplasmic mediator activity and molecular function this GO :0031016 and pancreas development and biological process this GO :0031053 and primary miRNA processing and biological process this GO :0031625 and ubiquitin protein ligase binding and molecular function this GO :0032444 and activin responsive factor complex and cellular component this GO :0032924 and activin receptor signaling pathway and biological process this GO :0033613 and activating transcription factor binding and molecular function this GO :0034713 and type I transforming growth factor beta receptor binding and molecular function this GO :0035265 and organ growth and biological process this GO :0035326 and enhancer binding and molecular function this GO :0035556 and intracellular signal transduction and biological process this GO :0038092 and nodal signaling pathway and biological process this GO :0043234 and protein complex and cellular component this GO :0045165 and cell fate commitment and biological process this GO :0045892 and negative regulation of transcription, this GO :0000122 and negative regulation of transcription from RNA polymerase II promoter and biological process this GO :0000790 and nuclear chromatin and cellular component this GO :0001657 and ureteric bud development and biological process this GO :0001701 and in utero embryonic development and biological process this GO :0001706 and endoderm formation and biological process this GO :0001707 and mesoderm formation and biological process this GO :0003677 and DNA binding and molecular function this GO :0003682 and chromatin binding and molecular function this GO :0003690 and double-stranded DNA binding and molecular function this GO :0003700 and sequence-specific DNA binding transcription factor activity and molecular function this GO :0005160 and transforming growth factor beta receptor binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005622 and intracellular and cellular component this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0005667 and transcription factor complex and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0006351 and transcription, this GO :0003677: DNA binding, this GO :0003677: DNA binding and also this GO :0003682: chromatin binding and also this GO :0003690: double-stranded DNA binding and also this GO :0003700: sequence-specific DNA binding transcription factor activity and also this GO :0005160: transforming growth factor beta receptor binding and also this GO :0005515: protein binding and also this GO :0008134: transcription factor binding and also this GO :0019902: phosphatase binding and also this GO :0030618: transforming growth factor beta receptor, this GO :0003682: chromatin binding, this GO :0003690: double-stranded DNA binding, this GO :0003700: sequence-specific DNA binding transcription factor activity, this GO :0005160: transforming growth factor beta receptor binding, this GO :0005515: protein binding, this GO :0008134: transcription factor binding, this GO :0019902: phosphatase binding, this GO :0030618: transforming growth factor beta receptor, this GO :0031625: ubiquitin protein ligase binding, this GO :0033613: activating transcription factor binding, this GO :0034713: type I transforming growth factor beta receptor binding, this GO :0035326: enhancer binding, this GO :0046332: SMAD binding, this GO :0046872: metal ion binding, this GO :0070410: co-SMAD binding, this GO :0070411: I-SMAD binding, this GO :0070412: R-SMAD binding, transforming growth factor beta receptor, 516010, SMAD family member 2 |
| Identity | 6768 |
| Gene | SMAD2 |
| Long gene name | SMAD family member 2 |
| Synonyms gene | MADH2 |
| Synonyms gene name | MAD, mothers against DPP homolog 2 (Drosophila) , mothers against decapentaplegic homolog 2 (Drosophila) SMAD |
| Synonyms | MADR2 JV18-1 |
| Locus | 18q21, 1 |
| Discovery year | 1996-11-15 |
| GenBank acession | U65019 |
| Entrez gene record | 4087 |
| Pubmed identfication | 8752209 8673135 |
| RefSeq identity | NM_005901 |
| Classification | SMAD family |
| Havana BLAST/BLAT | OTTHUMG00000132652 |
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