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SMAD3 Antibody

SMAD3 Antibody size: 0.08 ml 199

Supplier NJS POLY
Price 199
Size 0.08 ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
CategoryAntibody
Concentration pH 7, with 0, 09% sodium azide, 4, In 1X PBS
FormAntigen affinity purified
ConjugationUnconjugated
ClonePolyclonal antibody
Recognised antigenSMAD3
Host animalRabbit (Oryctolagus cuniculus)
ClonalityPolyclonal (rabbit origin)
Species reactivity Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens)
Tested applications ELISA, IF, IHC, WB
Recommended dilutionsIHC (Paraffin): 1:10-1:50, Immunofluorescence: 1:10-1:50, Western blot: 1:1000
NotesTitration of the SMAD3 antibody may be required due to differences in protocols and secondary/substrate sensitivity
Intented useThis SMAD3 antibodyis to be used only for research purposes and not for diagnostics
Uniprot #P84022
PurityAntigen affinity
Description Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription, Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF- mediated chemotaxis of monocytes, Positively regulates PDPK1 kinase activity by stimulating its dissociation from the&, Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures, This effect on wound healing appears to be hormone-sensitive, [UniProt], activates transcription, on formation of the&, &, &, &, <, <, <, (transforming growth factor) and activin type 1 receptor kinases, /<, /a>, /a>, /a>, /a>, /search_result, /search_result, /search_result, /search_result, 14-3-3<, SMAD3 is a receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by&, SMAD3<, SMAD4<, TGF-beta<, a href=, a href=, a href=, a href=, complex, nbsp, nbsp, nbsp, nbsp, nbsp, nbsp, php?search_txt=14-3-3>, php?search_txt=smad3>, php?search_txt=smad4>, php?search_txt=tgfb>, protein YWHAQ which acts as a negative regulator
ImmunogenA portion of amino acids 127-156 from the human protein was used as the immunogen for this SMAD3 antibody
Storage Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the SMAD3 antibody and store frozen at -20 deg
PropertiesC, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°
Gene targetSMAD3
Short nameSMAD3 Antibody
Technique antibodies against human proteins, antibodies for, Antibody
Alternative nameSMAD family member 3 (Antibody to)
Alternative techniqueantibodies
Alternative to gene target DNA-templated and biological process this GO :0006355 and regulation of transcription, DNA-templated and biological process this GO :0006367 and transcription initiation from RNA polymerase II promoter and biological process this GO :0006810 and transport and biological process this GO :0006919 and activation of cysteine-type endopeptidase activity involved in apoptotic process and biological process this GO :0006955 and immune response and biological process this GO :0007050 and cell cycle arrest and biological process this GO :0007179 and transforming growth factor beta receptor signaling pathway and biological process this GO :0007183 and SMAD protein complex assembly and biological process this GO :0007369 and gastrulation and biological process this GO :0007492 and endoderm development and biological process this GO :0008013 and beta-catenin binding and molecular function this GO :0008134 and transcription factor binding and molecular function this GO :0008270 and zinc ion binding and molecular function this GO :0008285 and negative regulation of cell proliferation and biological process this GO :0009880 and embryonic pattern specification and biological process this GO :0010467 and gene expression and biological process this GO :0010694 and positive regulation of alkaline phosphatase activity and biological process this GO :0010718 and positive regulation of epithelial to mesenchymal transition and biological process this GO :0016202 and regulation of striated muscle tissue development and biological process this GO :0017015 and regulation of transforming growth factor beta receptor signaling pathway and biological process this GO :0019049 and evasion or tolerance of host defenses by virus and biological process this GO :0019899 and enzyme binding and molecular function this GO :0019901 and protein kinase binding and molecular function this GO :0019902 and phosphatase binding and molecular function this GO :0023019 and signal transduction involved in regulation of gene expression and biological process this GO :0030308 and negative regulation of cell growth and biological process this GO :0030335 and positive regulation of cell migration and biological process this GO :0030501 and positive regulation of bone mineralization and biological process this GO :0030512 and negative regulation of transforming growth factor beta receptor signaling pathway and biological process this GO :0030618 and transforming growth factor beta receptor, DNA-templated and biological process this GO :0045930 and negative regulation of mitotic cell cycle and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0046332 and SMAD binding and molecular function this GO :0048340 and paraxial mesoderm morphogenesis and biological process this GO :0048589 and developmental growth and biological process this GO :0048617 and embryonic foregut morphogenesis and biological process this GO :0048701 and embryonic cranial skeleton morphogenesis and biological process this GO :0050678 and regulation of epithelial cell proliferation and biological process this GO :0050728 and negative regulation of inflammatory response and biological process this GO :0050776 and regulation of immune response and biological process this GO :0050821 and protein stabilization and biological process this GO :0050927 and positive regulation of positive chemotaxis and biological process this GO :0051098 and regulation of binding and biological process this GO :0051496 and positive regulation of stress fiber assembly and biological process this GO :0051894 and positive regulation of focal adhesion assembly and biological process this GO :0060039 and pericardium development and biological process this GO :0060290 and transdifferentiation and biological process this GO :0061045 and negative regulation of wound healing and biological process this GO :0070306 and lens fiber cell differentiation and biological process this GO :0070410 and co-SMAD binding and molecular function this GO :0070412 and R-SMAD binding and molecular function this GO :0071141 and SMAD protein complex and cellular component this GO :0071144 and SMAD2-SMAD3 protein complex and cellular component this GO :0090263 and positive regulation of canonical Wnt signaling pathway and biological process this GO :0097191 and extrinsic apoptotic signaling pathway and biological process this GO :0097296 and activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway and biological process this GO :1901313 and positive regulation of gene expression involved in extracellular matrix organization and biological process, HSPC193 and HsT17436 and JV15-2 and LDS1C and LDS3 and MADH3, SMAD3 and IDBG-18366 and ENSG00000166949 and 4088, SMAD3 and IDBG-645466 and ENSBTAG00000012599 and 515125, Smad3 and IDBG-175693 and ENSMUSG00000032402 and 17127, nuclei, pathway-specific cytoplasmic mediator activity, pathway-specific cytoplasmic mediator activity and also this GO :0031490: chromatin DNA binding and also this GO :0031625: ubiquitin protein ligase binding and also this GO :0035326: enhancer binding and also this GO :0042803: protein homodimerization activity and also this GO :0043130: ubiquitin binding and also this GO :0043425: bHLH transcription factor binding and also this GO :0043565: sequence-specific DNA binding and also this GO :0044212: transcription regulatory region DNA binding and also this GO :0046332: SMAD binding and also this GO :0070410: co-SMAD binding and also this GO :0070412: R-SMAD binding, pathway-specific cytoplasmic mediator activity and molecular function this GO :0030878 and thyroid gland development and biological process this GO :0031053 and primary miRNA processing and biological process this GO :0031490 and chromatin DNA binding and molecular function this GO :0031625 and ubiquitin protein ligase binding and molecular function this GO :0032332 and positive regulation of chondrocyte differentiation and biological process this GO :0032731 and positive regulation of interleukin-1 beta production and biological process this GO :0032909 and regulation of transforming growth factor beta2 production and biological process this GO :0032916 and positive regulation of transforming growth factor beta3 production and biological process this GO :0032924 and activin receptor signaling pathway and biological process this GO :0033689 and negative regulation of osteoblast proliferation and biological process this GO :0035326 and enhancer binding and molecular function this GO :0035413 and positive regulation of catenin import into nucleus and biological process this GO :0035556 and intracellular signal transduction and biological process this GO :0038092 and nodal signaling pathway and biological process this GO :0042060 and wound healing and biological process this GO :0042110 and T cell activation and biological process this GO :0042177 and negative regulation of protein catabolic process and biological process this GO :0042803 and protein homodimerization activity and molecular function this GO :0042993 and positive regulation of transcription factor import into nucleus and biological process this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0043130 and ubiquitin binding and molecular function this GO :0043234 and protein complex and cellular component this GO :0043235 and receptor complex and cellular component this GO :0043425 and bHLH transcription factor binding and molecular function this GO :0043565 and sequence-specific DNA binding and molecular function this GO :0044212 and transcription regulatory region DNA binding and molecular function this GO :0045087 and innate immune response and biological process this GO :0045216 and cell-cell junction organization and biological process this GO :0045668 and negative regulation of osteoblast differentiation and biological process this GO :0045893 and positive regulation of transcription, this GO :0000122 and negative regulation of transcription from RNA polymerase II promoter and biological process this GO :0000790 and nuclear chromatin and cellular component this GO :0000987 and core promoter proximal region sequence-specific DNA binding and molecular function this GO :0000988 and protein binding transcription factor activity and molecular function this GO :0001102 and RNA polymerase II activating transcription factor binding and molecular function this GO :0001501 and skeletal system development and biological process this GO :0001649 and osteoblast differentiation and biological process this GO :0001657 and ureteric bud development and biological process this GO :0001666 and response to hypoxia and biological process this GO :0001701 and in utero embryonic development and biological process this GO :0001707 and mesoderm formation and biological process this GO :0001756 and somitogenesis and biological process this GO :0001889 and liver development and biological process this GO :0001933 and negative regulation of protein phosphorylation and biological process this GO :0001947 and heart looping and biological process this GO :0002076 and osteoblast development and biological process this GO :0002520 and immune system development and biological process this GO :0003677 and DNA binding and molecular function this GO :0003682 and chromatin binding and molecular function this GO :0003690 and double-stranded DNA binding and molecular function this GO :0003700 and sequence-specific DNA binding transcription factor activity and molecular function this GO :0005160 and transforming growth factor beta receptor binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005518 and collagen binding and molecular function this GO :0005622 and intracellular and cellular component this GO :0005634 and nucleus and cellular component this GO :0005637 and nuclear inner membrane and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0005667 and transcription factor complex and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006351 and transcription, this GO :0000987: core promoter proximal region sequence-specific DNA binding, this GO :0000987: core promoter proximal region sequence-specific DNA binding and also this GO :0000988: protein binding transcription factor activity and also this GO :0001102: RNA polymerase II activating transcription factor binding and also this GO :0003677: DNA binding and also this GO :0003682: chromatin binding and also this GO :0003690: double-stranded DNA binding and also this GO :0003700: sequence-specific DNA binding transcription factor activity and also this GO :0005160: transforming growth factor beta receptor binding and also this GO :0005515: protein binding and also this GO :0005518: collagen binding and also this GO :0008013: beta-catenin binding and also this GO :0008134: transcription factor binding and also this GO :0008270: zinc ion binding and also this GO :0019899: enzyme binding and also this GO :0019901: protein kinase binding and also this GO :0019902: phosphatase binding and also this GO :0030618: transforming growth factor beta receptor, this GO :0000988: protein binding transcription factor activity, this GO :0001102: RNA polymerase II activating transcription factor binding, this GO :0003677: DNA binding, this GO :0003682: chromatin binding, this GO :0003690: double-stranded DNA binding, this GO :0003700: sequence-specific DNA binding transcription factor activity, this GO :0005160: transforming growth factor beta receptor binding, this GO :0005515: protein binding, this GO :0005518: collagen binding, this GO :0008013: beta-catenin binding, this GO :0008134: transcription factor binding, this GO :0008270: zinc ion binding, this GO :0019899: enzyme binding, this GO :0019901: protein kinase binding, this GO :0019902: phosphatase binding, this GO :0030618: transforming growth factor beta receptor, this GO :0031490: chromatin DNA binding, this GO :0031625: ubiquitin protein ligase binding, this GO :0035326: enhancer binding, this GO :0042803: protein homodimerization activity, this GO :0043130: ubiquitin binding, this GO :0043425: bHLH transcription factor binding, this GO :0043565: sequence-specific DNA binding, this GO :0044212: transcription regulatory region DNA binding, this GO :0046332: SMAD binding, this GO :0070410: co-SMAD binding, this GO :0070412: R-SMAD binding, transforming growth factor beta receptor, SMAD family member 3
Identity 6769
Gene SMAD3
Long gene name SMAD family member 3
Synonyms gene MADH3
Synonyms gene name MAD, mothers against DPP homolog 3 (Drosophila) , mothers against decapentaplegic homolog 3 (Drosophila) SMAD
Synonyms JV15-2 HsT17436
Locus 15q22, 33
Discovery year 1996-11-15
GenBank acession BC050743
Entrez gene record 4088
Pubmed identfication 8774881 8673135
RefSeq identity NM_005902
Classification SMAD family
Havana BLAST/BLAT OTTHUMG00000133230
Locus Specific Databases The Globin Gene Server

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