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Smad3 (Ser423) Antibody, Biotin Conjugated

Smad3 (Ser423) Antibody, Biotin Conjugated size: 0.1ml 350

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 350
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name Biotin Conjugated, Smad3 (Ser423) Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationBiotinylated
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenSmad3 Ser423
SpecificityThis is a highly specific antibody against Smad3 Ser423
ModificationPhosphorylation
Modification siteSer423
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic phosphopeptide derived from human Smad3 around the phosphorylation site of Ser423
Gene ID number4088
Tested applications IHC-P, WB
Recommended dilutions IHC-P(1:100-500), WB(1:100-1000)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background (Smad4) that functions via its interaction to the various Smads, (Smad6 and 7), 5, Activated Smad3 oligomerizes with Smad4 upon TGF beta stimulation and translocates as a complex into the nucleus, Phosphorylation of the two TGF beta dependent serines 423 and 425 in the C terminus of Smad3 is critical for Smad3 transcriptional activity and TGF beta signaling, The Smad family is divided into three subclasses: receptor regulated Smads, activin/TGF beta receptor regulated (Smad2 and 3) or BMP receptor regulated (Smad 1, allowing its binding to DNA and transcription factors, and 8), and the inhibitory Smads, differentiation and development, the common partner, Smad3 is a 50 kDa member of a family of proteins that act as key mediators of TGF beta superfamily signaling in cell proliferation
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation EmissionN/A
Synonyms HSPC193, JV15 2, JV152, MAD mothers against decapentaplegic Drosophila homolog 3, MAD3, MADH 3, MADH3, Mothers against DPP homolog 3, Mothers against decapentaplegic homolog 3, SMA and MAD related protein 3, SMAD, SMAD 3, SMAD-3, SMAD3_HUMAN, Smad3 phospho S423, hMAD 3, hSMAD3, p-Smad3 Ser423, Smad3 phospho Ser423
Properties Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetSmad3 (Ser423) Conjugated
Short name Conjugated, Smad3 (Ser423) Antibody
Technique antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelBiotin
Alternative name biotinilated coupled, SMAD family member 3 (Ser423) (Antibody to)
Alternative techniqueantibodies

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