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SMURF2 Antibody, Biotin Conjugated

SMURF2 Antibody, Biotin Conjugated size: 0.1ml 333

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 333
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name Biotin Conjugated, SMURF2 Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationBiotinylated
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenSMURF2
SpecificityThis is a highly specific antibody against SMURF2
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen_range615-665/748
Subcellular location Intracellular, Nucleus
SourceKLH conjugated synthetic peptide derived from human SMURF2
Gene ID number64750
Swiss ProtQ9HAU4
Tested applications IHC-P, WB
Recommended dilutions IHC-P(1:100-500), WB(1:100-1000)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level, Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2, Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3, In addition, In this way, Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation, SMAD2 may recruit substrates, for ubiquitin-mediated degradation, interaction with SMAD7 activates autocatalytic degradation, such as SNON, which is prevented by interaction with SCYE1, E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation EmissionN/A
Properties Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetSMURF2 Conjugated
Short name Conjugated, SMURF2 Antibody
Technique antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelBiotin
Alternative name biotinilated coupled, SMAD specific E3 ubiquitin protein ligase 2 (Antibody to)
Alternative techniqueantibodies
Alternative to gene target DNA-templated and biological process this GO :0006367 and transcription initiation from RNA polymerase II promoter and biological process this GO :0006511 and ubiquitin-dependent protein catabolic process and biological process this GO :0007179 and transforming growth factor beta receptor signaling pathway and biological process this GO :0010467 and gene expression and biological process this GO :0016567 and protein ubiquitination and biological process this GO :0016874 and ligase activity and molecular function this GO :0017015 and regulation of transforming growth factor beta receptor signaling pathway and biological process this GO :0030509 and BMP signaling pathway and biological process this GO :0030512 and negative regulation of transforming growth factor beta receptor signaling pathway and biological process this GO :0030579 and ubiquitin-dependent SMAD protein catabolic process and biological process this GO :0042787 and protein ubiquitination involved in ubiquitin-dependent protein catabolic process and biological process this GO :0042802 and identical protein binding and molecular function this GO :0045121 and membrane raft and cellular component this GO :0045892 and negative regulation of transcription, DNA-templated and biological process this GO :0046332 and SMAD binding and molecular function, SMAD binding, SMURF2 and IDBG-641903 and ENSBTAG00000019853 and 540826, SMURF2 and IDBG-64876 and ENSG00000108854 and 64750, Smurf2 and IDBG-213588 and ENSMUSG00000018363 and 66313, nuclei, this GO :0000122 and negative regulation of transcription from RNA polymerase II promoter and biological process this GO :0000151 and ubiquitin ligase complex and cellular component this GO :0004842 and ubiquitin-protein transferase activity and molecular function this GO :0005160 and transforming growth factor beta receptor binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006351 and transcription, this GO :0004842: ubiquitin-protein transferase activity, this GO :0004842: ubiquitin-protein transferase activity and also this GO :0005160: transforming growth factor beta receptor binding and also this GO :0005515: protein binding and also this GO :0016874: ligase activity and also this GO :0042802: identical protein binding and also this GO :0046332: SMAD binding, this GO :0005160: transforming growth factor beta receptor binding, this GO :0005515: protein binding, this GO :0016874: ligase activity, this GO :0042802: identical protein binding, this GO :0046332: SMAD binding, SMAD specific E3 ubiquitin protein ligase 2
Identity 16809
Gene SMURF2
Long gene name SMAD specific E3 ubiquitin protein ligase 2
Locus 17q23, 1 , 3-q24
Discovery year 2004-07-29
GenBank acession AF301463
Entrez gene record 64750
Pubmed identfication 11016919
RefSeq identity NM_022739
Classification C2 domain containing
Havana BLAST/BLAT OTTHUMG00000179189

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