| Long name | SPARC Primary Polyclonal Antibody |
| Also known as | SPARC PAb |
| Category | Primary Antibodies |
| Conjugation | Unconjugated |
| Target Antigen | SPARC |
| Specificity | This is a highly specific antibody against SPARC |
| Modification(s) | None |
| Modification site(s) | Unmodified antibody |
| Clonality | Polyclonal antibody |
| Clone number | Polyclonal antibody |
| Concentration | 1ug per 1ul |
| Immunogen range | 120-160/303 |
| Subcellular location | Secreted |
| Source | This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human SPARC |
| Gene ID number | 6678 |
| Swiss Prot | P09486 |
| Tested Applications | IF(IHC-P), IHC-P, WB |
| Recommended dilutions | IF(IHC-P)(1:50-200), IHC-P(1:100-500), WB(1:100-1000) |
| Cross reactivity | Mouse, Rat, Human |
| Cross reactive species details | not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the target antigen | Binds calcium and copper, PDGF and cell membranes, There are two calcium binding sites, albumin, an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity, several types of collagen, thrombospondin, Appears to regulate cell growth through interactions with the extracellular matrix and cytokines |
| Purification method | This antibody was purified via Protein A |
| Storage conditions | 50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to 1 year, Keep the antibody in aqueous buffered solution containing 1% BSA |
| Synonym names | BM-4, Basement-membrane protein 4, Osteonectin, SPARC, Secreted protein acidic and rich in cysteine, ON |
| Properties | C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24° |
| Gene target | SPARC |
| Short name | SPARC Antibody |
| Technique | antibodies against human proteins, antibodies for, Antibody |
| Alternative name | acidic, cysteine-rich (osteonectin) (Antibody to), secreted protein |
| Alternative technique | antibodies |
| Alternative to gene target | ON, SPARC and IDBG-54562 and ENSG00000113140 and 6678, SPARC and IDBG-646341 and ENSBTAG00000014835 and 282077, Sparc and IDBG-175174 and ENSMUSG00000018593 and 20692, acidic, cysteine-rich (osteonectin), extracellular matrix binding, nuclei, this GO :0001503 and ossification and biological process this GO :0002576 and platelet degranulation and biological process this GO :0005509 and calcium ion binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005518 and collagen binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005578 and proteinaceous extracellular matrix and cellular component this GO :0005604 and basement membrane and cellular component this GO :0005615 and extracellular space and cellular component this GO :0005622 and intracellular and cellular component this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0007165 and signal transduction and biological process this GO :0007507 and heart development and biological process this GO :0007596 and blood coagulation and biological process this GO :0009629 and response to gravity and biological process this GO :0010288 and response to lead ion and biological process this GO :0030168 and platelet activation and biological process this GO :0030198 and extracellular matrix organization and biological process this GO :0030324 and lung development and biological process this GO :0031012 and extracellular matrix and cellular component this GO :0031093 and platelet alpha granule lumen and cellular component this GO :0031988 and membrane-bounded vesicle and cellular component this GO :0032496 and response to lipopolysaccharide and biological process this GO :0033591 and response to L-ascorbic acid and biological process this GO :0034097 and response to cytokine and biological process this GO :0042060 and wound healing and biological process this GO :0042127 and regulation of cell proliferation and biological process this GO :0043434 and response to peptide hormone and biological process this GO :0045471 and response to ethanol and biological process this GO :0046686 and response to cadmium ion and biological process this GO :0048839 and inner ear development and biological process this GO :0050840 and extracellular matrix binding and molecular function this GO :0051384 and response to glucocorticoid and biological process this GO :0051591 and response to cAMP and biological process this GO :0051592 and response to calcium ion and biological process this GO :0060348 and bone development and biological process this GO :0071363 and cellular response to growth factor stimulus and biological process this GO :0071682 and endocytic vesicle lumen and cellular component, this GO :0005509: calcium ion binding, this GO :0005509: calcium ion binding and also this GO :0005515: protein binding and also this GO :0005518: collagen binding and also this GO :0050840: extracellular matrix binding, this GO :0005515: protein binding, this GO :0005518: collagen binding, this GO :0050840: extracellular matrix binding, secreted protein |
| Identity | 11219 |
| Gene | SPARC |
| Long gene name | secreted protein acidic and cysteine rich |
| Synonyms gene | ON |
| Synonyms gene name | osteonectin |
| Synonyms name | cysteine-rich protein secreted protein acidic and rich in cysteine |
| Locus | 5q33, 1 |
| Discovery year | 2001-06-22 |
| Entrez gene record | 6678 |
| Pubmed identfication | 2838412 3410046 |
| RefSeq identity | NM_003118 |
| Classification | SPARC family |
| Havana BLAST/BLAT | OTTHUMG00000130122 |
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