Name : SSEA4 Antibody, FITC Conjugated
Supplier : BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price :333
SKU : GEN3427258960
| Long name | FITC Conjugated, SSEA4 Polyclonal Antibody |
| Category | Conjugated Primary Antibodies |
| Conjugation | Anti-FITC Antibody |
| Host Organism | Rabbit (Oryctolagus cuniculus) |
| Target Antigen | SSEA4 |
| Specificity | This is a highly specific antibody against SSEA4 |
| Modification | Unmodified |
| Modification site | None |
| Clonality | Polyclonal |
| Clone | Polyclonal antibody |
| Concentration | 1ug per 1ul |
| Subcellular location | Extracellular |
| Source | Human embryonal carcinoma cell line 2102Ep full length protein |
| Tested applications | FCM |
| Recommended dilutions | FCM(1:20-100) |
| Crossreactivity | Human |
| Crossreactive species details | not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background | Human Embryonic Carcinoma Cells and Human Embryonic Germ Cells, Monoclonal antibodies to this target have been widely used in the characterization of pluripotent stem cells, Mouse pluripotent stem cells are not recognised by anti-SSEA-4 antibodies but do express the antigen upon differentiation, SSEA-4 can be used as a marker of Human Embryonic Stem Cells,  , SSEA-4 (Stage-specific embryonic antigen 4) is a glycoprotein expressed early in embryonic development and in pluripotent stem cells |
| Purification method | This antibody was purified via Protein A |
| Storage conditions | 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission | 494nm/518nm |
| Synonyms | Stage-specific embryonic antigen 4, SSEA-4 |
| Properties | Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target | SSEA4 Conjugated |
| Short name | FITC Conjugated, SSEA4 Antibody |
| Technique | Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label | FITC |
| Alternative name | fluorecein coupled, SSEA4 (Antibody to) |
| Alternative technique | antibodies |