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TFIID/TATA binding protein TBP Antibody, FITC Conjugated

TFIID/TATA binding protein TBP Antibody, FITC Conjugated size: 0.1ml 333

Price 333
Size 0.1ml
Long name FITC Conjugated, TFIID/TATA binding protein TBP Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenTFIID/TATA binding protein TBP
SpecificityThis is a highly specific antibody against TFIID/TATA binding protein TBP
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic peptide derived from human TATA binding protein TBP
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Binding of TFIID to the TATA element initiates assembly of the other factors into a pre-initiation complex, Such factors can be divided into two major functional classes: the basal factors that are required for transcription of all Pol II genes, TFIIB, TFIID, TFIIE, TFIIF and TFIIH, The TATA-binding subunit of TFIID (designated TFIIDt or TBP) from higher eukaryotes contains a highly conserved 180 amino acid C-terminal domain, The basal transcription factors and Pol II form a specific multiprotein complex near the transcription start site by interacting with core promotor elements such as the TATA box generally located 25-30 base pairs upstream of the transcription start site, and sequence-specific factors that regulate gene expression, including TFIIA, initiation of transcription from protein-coding genes is a complex process requiring RNA polymerase II and broad families of auxiliary transcription factors, In eukaryotic systems
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Synonyms TFIID
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetTFIID/TATA binding protein TBP Conjugated
Short name FITC Conjugated, TFIID/TATA binding protein TBP Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, TFIID/TATA binding protein TBP (Antibody to)
Alternative techniqueantibodies
Identity 11588
Gene TBP
Long gene name TATA-box binding protein
Synonyms gene GTF2D1 SCA17
Locus 6q27
Discovery year 1993-05-26
GenBank acession M55654
Entrez gene record 6908
Pubmed identfication 2194289 11448935
RefSeq identity NM_003194
Classification General transcription factors
Havana BLAST/BLAT OTTHUMG00000016084

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