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TOP1 (1291CT875.142.166) Monoclonal Antibody

TOP1 (1291CT875.142.166) Monoclonal Antibody size: 0.1ml 211

Supplier BIOSS PRIMARY UNCONJUGATED ANTIBODIES
Price 211
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name142, 166) Monoclonal Antibody, TOP1 (1291CT875
Also known as142, 166) Monoclonal Antibody, TOP1 (1291CT875
CategoryPrimary Antibodies
ConjugationUnconjugated
Target AntigenTOP1
SpecificityThis is a highly specific antibody against TOP1
Modification(s)None
Modification site(s)Unmodified antibody
ClonalityMonoclonal
Clone number1291CT875, 142, 166
SourceThis TOP1 antibody is generated from a mouse immunized with a recombinant protein from the N-terminal region of human TOP1
Gene ID number7150
Swiss ProtP11387
Tested Applications FCM, WB
Recommended dilutions FC(1:25), WB(1:1000)
Cross reactivity Rat, Human
Cross reactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Background of the target antigen Finally, Introduces a single-strand break via transesterification at a target site in duplex DNA, Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the ARNTL/BMAL1 promoter, Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells, The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils, The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, in the religation step, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity), Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex
Purification method This antibody is purified through a protein G column, followed by dialysis against PBS, 09% (W/V) sodium azide, Purified monoclonal antibody supplied in PBS with 0
Storage conditions 50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to 1 year, Keep the antibody in aqueous buffered solution containing 1% BSA
Synonym names DNA topoisomerase 1, DNA topoisomerase I, TOP1, TOPI
PropertiesC, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°
About Each murine monoclonal anibody has his own affinity specific for the clone, Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes, Monoclonals of this antigen are available in different clones
Gene target142, 166) Monoclonal, TOP1 (1291CT875
Short name142, 166) Monoclonal Antibody, TOP1 (1291CT875
Technique Monoclonals or monoclonal antibodies, antibodies against human proteins, antibodies for, Antibody
Alternative name142, 166) monoclonal (Antibody to), topoisomerase (Desoxyribonucleic acid) I (1291CT875
Alternative techniqueantibodies
Alternative to gene target BT, TOP1 and IDBG-75139 and ENSG00000198900 and 7150, TOPI, Top1 and IDBG-211615 and ENSMUSG00000070544 and 21969, nuclei, poly(A) RNA binding, this GO :0000228 and nuclear chromosome and cellular component this GO :0000932 and cytoplasmic mRNA processing body and cellular component this GO :0003677 and DNA binding and molecular function this GO :0003682 and chromatin binding and molecular function this GO :0003917 and DNA topoisomerase type I activity and molecular function this GO :0003918 and DNA topoisomerase (ATP-hydrolyzing) activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0005694 and chromosome and cellular component this GO :0005730 and nucleolus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0006200 and ATP catabolic process and biological process this GO :0006260 and DNA replication and biological process this GO :0006265 and DNA topological change and biological process this GO :0006338 and chromatin remodeling and biological process this GO :0007059 and chromosome segregation and biological process this GO :0012501 and programmed cell death and biological process this GO :0016032 and viral process and biological process this GO :0016310 and phosphorylation and biological process this GO :0031298 and replication fork protection complex and cellular component this GO :0040016 and embryonic cleavage and biological process this GO :0042493 and response to drug and biological process this GO :0043204 and perikaryon and cellular component this GO :0044822 and poly(A) RNA binding and molecular function, this GO :0003677: DNA binding, this GO :0003677: DNA binding and also this GO :0003682: chromatin binding and also this GO :0003917: DNA topoisomerase type I activity and also this GO :0003918: DNA topoisomerase (ATP-hydrolyzing) activity and also this GO :0005515: protein binding and also this GO :0044822: poly(A) RNA binding, this GO :0003682: chromatin binding, this GO :0003917: DNA topoisomerase type I activity, this GO :0003918: DNA topoisomerase (ATP-hydrolyzing) activity, this GO :0005515: protein binding, this GO :0044822: poly(A) RNA binding, 39255 and IDBG-642660 and ENSBTAG00000007960 and 534799, topoisomerase (DNA) I
Identity 11986
Gene TOP1
Long gene name topoisomerase (DNA) I
Locus 20q12
Discovery year 1989-02-23
Entrez gene record 7150
Classification Topoisomerases
Havana BLAST/BLAT OTTHUMG00000033057

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