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Topoisomerase II alpha Antibody

Topoisomerase II alpha Antibody size: 0.1mg 389

Supplier NJS POLY
Price 389
Size 0.1mg
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
CategoryAntibody
Concentration2ml sterile DI water, 5mg/ml if reconstituted with 0
FormAntigen affinity purified
ConjugationUnconjugated
ClonePolyclonal antibody
Recognised antigenTopoisomerase II alpha
Host animalRabbit (Oryctolagus cuniculus)
ClonalityPolyclonal (rabbit origin)
Species reactivity Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens)
Tested applicationsWB
Recommended dilutions5-1ug/ml, Western blot: 0
Notes Titration of the Topoisomerase II alpha antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts
Intented useThis Topoisomerase II alpha antibodyis to be used only for research purposes and not for diagnostics
Uniprot #P11388
PurityAntigen affinity
Description Addition of purified topoisomerase II-alpha reconstituted chromatin-dependent activation activity in reactions with core pol II, DNA topoisomerase II-alpha is associated with the pol II holoenzyme and is a required component of chromatin-dependent coactivation, DNA topoisomerases are enzymes that control and alter the topologic states of DNA in both prokaryotes and eukaryotes, Furthermore, It appears likely that the reaction catalyzed by topoisomerase II involves the crossing-over of 2 DNA segments, Specific inhibitors of topoisomerase II blocked transcription on chromatin templates, The TOP2A gene spans approximately 30 kb and contains 35 exons, There are about 100, Topoisomerase II from eukaryotic cells catalyzes the relaxation of supercoiled DNA molecules, and unknotting of circular DNA, but did not affect transcription on naked templates, catenation, constituting about 0, decatenation, knotting, 000 molecules of topoisomerase II per HeLa cell nucleus, 1% of the nuclear extract, The human topoisomerase II enzyme is encoded by a single-copy gene which is mapped to 17q21-q22
ImmunogenAn amino acid sequence from the C-terminus of human Topoisomerase II alpha (RKPSTSDDSDSNFEK) was used as the immunogen for this Topoisomerase II alpha antibody
Storage Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the Topoisomerase II alpha antibody can be stored for up to one month at 4oC, After reconstitution
PropertiesC, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°
Gene targetTopoisomerase II alpha
Short nameTopoisomerase II alpha Antibody
Technique antibodies against human proteins, antibodies for, Antibody
Alternative nameTopoisomerase II a (Antibody to)
Alternative techniqueantibodies
Identity 11989
Gene TOP2A
Long gene name topoisomerase (DNA) II alpha
Synonyms gene TOP2
Synonyms gene name topoisomerase (DNA) II alpha 170kDa
Locus 17q21, 2
Discovery year 1989-03-23
Entrez gene record 7153
Classification Topoisomerases
Havana BLAST/BLAT OTTHUMG00000155008

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