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TRIM25(Ser100) Antibody, FITC Conjugated

TRIM25(Ser100) Antibody, FITC Conjugated size: 0.1ml 350

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 350
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name FITC Conjugated, TRIM25(Ser100) Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenTRIM25 Ser100
SpecificityThis is a highly specific antibody against TRIM25 Ser100
ModificationPhosphorylation
Modification siteSer100
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic phosphopeptide derived from human TRIM25 around the phosphorylation site of Ser100
Gene ID number7706
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Expression of the gene is upregulated in response to estrogen, The TRIM motif includes three zinc binding domains, The presence of potential DNA binding and dimerization transactivation domains suggests that this protein may act as a transcription factor, The protein localizes to the cytoplasm, a B box type 1 and a B box type 2, a RING, and a coiled coil region, and it is thought to mediate estrogen actions in breast cancer as a primary response gene, similar to several other members of the TRIM family, The protein encoded by this gene is a member of the tripartite motif (TRIM) family
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Synonyms EFP RNF147
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetTRIM25(Ser100) Conjugated
Short name FITC Conjugated, TRIM25(Ser100) Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, TRIM25(Ser100) (Antibody to)
Alternative techniqueantibodies
Identity 12932
Gene TRIM25
Long gene name tripartite motif containing 25
Synonyms gene ZNF147
Synonyms gene name zinc finger protein 147 (estrogen-responsive finger protein) tripartite motif-containing 25
Locus 17q23, 1
Discovery year 1994-03-16
GenBank acession D21205
Entrez gene record 7706
Pubmed identfication 7789997
RefSeq identity NM_005082
Classification Tripartite motif containing Ring finger proteins
Havana BLAST/BLAT OTTHUMG00000178124

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