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TRIM25(Thr91) Antibody, Biotin Conjugated

TRIM25(Thr91) Antibody, Biotin Conjugated size: 0.1ml 350

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 350
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name Biotin Conjugated, TRIM25(Thr91) Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationBiotinylated
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenTRIM25 Thr91
SpecificityThis is a highly specific antibody against TRIM25 Thr91
ModificationPhosphorylation
Modification siteThr91
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic phosphopeptide derived from human TRIM25 around the phosphorylation site of Thr91
Gene ID number7706
Tested applications IHC-P, WB
Recommended dilutions IHC-P(1:100-500), WB(1:100-1000)
CrossreactivityHuman
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Expression of the gene is upregulated in response to estrogen, The TRIM motif includes three zinc binding domains, The presence of potential DNA binding and dimerization transactivation domains suggests that this protein may act as a transcription factor, The protein localizes to the cytoplasm, a B box type 1 and a B box type 2, a RING, and a coiled coil region, and it is thought to mediate estrogen actions in breast cancer as a primary response gene, similar to several other members of the TRIM family, The protein encoded by this gene is a member of the tripartite motif (TRIM) family
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation EmissionN/A
Synonyms EFP RNF147
Properties Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetTRIM25(Thr91) Conjugated
Short name Conjugated, TRIM25(Thr91) Antibody
Technique antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelBiotin
Alternative name biotinilated coupled, TRIM25(Thr91) (Antibody to)
Alternative techniqueantibodies
Identity 12932
Gene TRIM25
Long gene name tripartite motif containing 25
Synonyms gene ZNF147
Synonyms gene name zinc finger protein 147 (estrogen-responsive finger protein) tripartite motif-containing 25
Locus 17q23, 1
Discovery year 1994-03-16
GenBank acession D21205
Entrez gene record 7706
Pubmed identfication 7789997
RefSeq identity NM_005082
Classification Tripartite motif containing Ring finger proteins
Havana BLAST/BLAT OTTHUMG00000178124

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