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UBE2E2 Antibody, FITC Conjugated

UBE2E2 Antibody, FITC Conjugated size: 0.1ml 333

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 333
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name FITC Conjugated, UBE2E2 Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenUBE2E2
SpecificityThis is a highly specific antibody against UBE2E2
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic peptide derived from human UBE2E2
Gene ID number7325
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background A wide range of enzymes facilitate this proteolytic ubiquitin pathway, The E3 enzyme then catalyzes the transfer of ubiquitin from E2 to the appropriate protein substrate, The first step in the ubiquitination process requires the ATP-dependent activation of the ubiquitin C-terminus and the assembly of multi-ubiquitin chains by the E1 enzyme, The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), The ubiquitin chain is then conjugated to the E2 enzyme to generate an intermediate ubiquitin-E2 complex, one of which is UBE2E2 (also known as UBCH8 in human), the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s), thereby playing an important role in protein degradation, thereby targeting that substrate for degradation, which functions as an E2 enzyme and catalyzes the ATP-dependent covalent attachment of ubiquitin to target proteins, Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Synonyms UbcH8 FLJ25157
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetUBE2E2 Conjugated
Short name FITC Conjugated, UBE2E2 Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, UBE2E2 (Antibody to)
Alternative techniqueantibodies
Identity 12478
Gene UBE2E2
Long gene name ubiquitin conjugating enzyme E2 E2
Synonyms gene name ubiquitin-conjugating enzyme E2E 2 (homologous to yeast UBC4/5) ubiquitin-conjugating enzyme E2E 2 (UBC4/5 homolog, yeast) ubiquitin-conjugating enzyme E2E 2
Locus 3p24, 3
Discovery year 1997-12-15
GenBank acession AK057886
Entrez gene record 7325
Pubmed identfication 9371400
RefSeq identity NM_152653
Classification Ubiquitin conjugating enzymes E2
Havana BLAST/BLAT OTTHUMG00000130482

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