Name : Ubiquitin Antibody, Biotin Conjugated
Supplier : BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price :333
SKU : GEN7958289196
| Long name | Biotin Conjugated, Ubiquitin Polyclonal Antibody |
| Category | Conjugated Primary Antibodies |
| Conjugation | Biotinylated |
| Host Organism | Rabbit (Oryctolagus cuniculus) |
| Target Antigen | Ubiquitin |
| Specificity | This is a highly specific antibody against Ubiquitin |
| Modification | Unmodified |
| Modification site | None |
| Clonality | Polyclonal |
| Clone | Polyclonal antibody |
| Concentration | 1ug per 1ul |
| Immunogen_range | 5-55/685 |
| Subcellular location | Nucleus, Cytoplasm |
| Source | KLH conjugated synthetic peptide derived from human Ubiquitin |
| Gene ID number | 7316 |
| Swiss Prot | P0CG48 |
| Tested applications | IHC-P, WB |
| Recommended dilutions | IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity | Mouse, Pig, Rat, Human |
| Crossreactive species details | not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background | DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B, Linear polymer chains formed via attachment by the initiator Met lead to cell signaling, Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation, Lys-29-linked is involved in lysosomal degradation, Lys-33-linked is involved in kinase modification, Lys-48-linked is involved in protein degradation via the proteasome, Lys-63-linked is involved in endocytosis, Polyubiquitin chains, Ubiquitin is usually conjugated to Lys residues of target proteins, When covalently bound, When polyubiquitin is free (unanchored-polyubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains), and in signaling, conjugation to Cys or Ser residues has been observed, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair, however, in rare cases, it also has distinct roles, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), or free (unanchored), such as in activation of protein kinases, when attached to a target protein, Ubiquitin: Exists either covalently attached to another protein |
| Purification method | This antibody was purified via Protein A |
| Storage conditions | 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission | N/A |
| Synonyms | Polyubiquitin-C, UBC, HMG2 |
| Properties | Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target | Ubiquitin Conjugated |
| Short name | Conjugated, Ubiquitin Antibody |
| Technique | antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label | Biotin |
| Alternative name | biotinilated coupled, Ubiquitin (Antibody to) |
| Alternative technique | antibodies |