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Ubiquitin Antibody, FITC Conjugated

Ubiquitin Antibody, FITC Conjugated size: 0.1ml 333

Price 333
Size 0.1ml
Long name FITC Conjugated, Ubiquitin Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenUbiquitin
SpecificityThis is a highly specific antibody against Ubiquitin
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen_range5-55/685
Subcellular location Nucleus, Cytoplasm
SourceKLH conjugated synthetic peptide derived from human Ubiquitin
Gene ID number7316
Swiss ProtP0CG48
Tested applications IF(ICC), IF(IHC-P)
Recommended dilutions IF(ICC)(1:50-200), IF(IHC-P)(1:50-200)
Crossreactivity Mouse, Pig, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B, Linear polymer chains formed via attachment by the initiator Met lead to cell signaling, Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation, Lys-29-linked is involved in lysosomal degradation, Lys-33-linked is involved in kinase modification, Lys-48-linked is involved in protein degradation via the proteasome, Lys-63-linked is involved in endocytosis, Polyubiquitin chains, Ubiquitin is usually conjugated to Lys residues of target proteins, When covalently bound, When polyubiquitin is free (unanchored-polyubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains), and in signaling, conjugation to Cys or Ser residues has been observed, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair, however, in rare cases, it also has distinct roles, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), or free (unanchored), such as in activation of protein kinases, when attached to a target protein, Ubiquitin: Exists either covalently attached to another protein
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Synonyms Polyubiquitin-C, UBC, HMG2
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetUbiquitin Conjugated
Short name FITC Conjugated, Ubiquitin Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, Ubiquitin (Antibody to)
Alternative techniqueantibodies

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