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Ubiquitin Protein Ligase E3A (UBE3A) ELISA kit

Ubiquitin Protein Ligase E3A (UBE3A) ELISA kit size: 1 plate of 96 wells 783

Price 783
Size 1 plate of 96 wells
Antigen detectable with this ELISA kitUbiquitin Protein Ligase E3A (UBE3A)
Species reactivityHomo sapiens (Human)
Range of detection 0, 0, 1, 10ng/mL, 2, 5ng/mL, 25ng/mL, 313-20ng/mL The standard curve concentrations used for the ELISA&rsquo, 313ng/mL, 5ng/mL, 625ng/mL, s were 20ng/mL
Sensitivity102ng/mL, The minimum detectable dose of this kit is typically less than 0
ApplicationELISA
Sample types tested for this ELISA kitTissue homogenates and other biological fluids
Time for performing t hourse test4, 5 hoursours
Test principle 10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ubiquitin Protein Ligase E3A (UBE3A), The concentration of Ubiquitin Protein Ligase E3A (UBE3A) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquitin Protein Ligase E3A (UBE3A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Ubiquitin Protein Ligase E3A (UBE3A), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn
Storage and handling Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt
Advisory Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes
Properties E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
TestELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Gene targetUbiquitin Protein Ligase E3A (UBE3A)
Short nameUbiquitin Protein Ligase E3A (UBE3A) ELISA kit
Technique &hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit
Hostantigen assay ELISA kit 1 Kit (96 Wells)
Alternative nameUbiquitin Protein Ligase E3A (ubiquitin protein ligase E3A) Enzyme-linked immunosorbent assay reagent
Alternative techniqueelisas
Alternative to gene target ANCR and AS and E6-AP and EPVE6AP and HPVE6A, UBE3A and IDBG-4274 and ENSG00000114062 and 7337, UBE3A and IDBG-628537 and ENSBTAG00000002487 and 100335247, Ube3a and IDBG-190437 and ENSMUSG00000025326 and 22215, ligase activity, nuclei, this GO :0000502 and proteasome complex and cellular component this GO :0001541 and ovarian follicle development and biological process this GO :0003713 and transcription coactivator activity and molecular function this GO :0004842 and ubiquitin-protein transferase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0006508 and proteolysis and biological process this GO :0006511 and ubiquitin-dependent protein catabolic process and biological process this GO :0007420 and brain development and biological process this GO :0014068 and positive regulation of phosphatidylinositol 3-kinase signaling and biological process this GO :0016032 and viral process and biological process this GO :0016567 and protein ubiquitination and biological process this GO :0016874 and ligase activity and molecular function this GO :0030521 and androgen receptor signaling pathway and biological process this GO :0035037 and sperm entry and biological process this GO :0042787 and protein ubiquitination involved in ubiquitin-dependent protein catabolic process and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0051865 and protein autoubiquitination and biological process this GO :0060736 and prostate gland growth and biological process this GO :0070936 and protein K48-linked ubiquitination and biological process, this GO :0003713: transcription coactivator activity, this GO :0003713: transcription coactivator activity and also this GO :0004842: ubiquitin-protein transferase activity and also this GO :0005515: protein binding and also this GO :0016874: ligase activity, this GO :0004842: ubiquitin-protein transferase activity, this GO :0005515: protein binding, this GO :0016874: ligase activity, 533136, ubiquitin protein ligase E3A
Identity 12496
Gene UBE3A
Long gene name ubiquitin protein ligase E3A
Synonyms gene EPVE6AP HPVE6A
Synonyms gene name human papilloma virus E6-associated protein
Synonyms AS ANCR E6-AP FLJ26981
Synonyms name Angelman syndrome
Locus 15q11, 2
Discovery year 1993-10-21
GenBank acession AF002224
Entrez gene record 7337
Pubmed identfication 8221889
RefSeq identity NM_000462
Havana BLAST/BLAT OTTHUMG00000171548
Locus Specific Databases Manchester LOVD LRG_15 , NGRL

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