| Antigen detectable with this ELISA kit | Vascular Endothelial Growth Factor B (VEGFB) |
| Species reactivity | Homo sapiens (Human) |
| Range of detection | 125pg/mL, 15, 250pg/mL, 31, 500pg/mL, 15, 62, 25pg/mL, 5pg/mL, 625-1000pg/mL The standard curve concentrations used for the ELISA&rsquo, 625pg/mL, s were 1000pg/mL |
| Sensitivity | 5pg/mL, The minimum detectable dose of this kit is typically less than 5 |
| Application | ELISA |
| Sample types tested for this ELISA kit | cell culture supernates and other biological fluids, cell lysates, plasma, tissue homogenates, Serum |
| Time for performing t hourse test | 4, 5 hoursours |
| Test principle | 10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vascular Endothelial Growth Factor B (VEGFB), The concentration of Vascular Endothelial Growth Factor B (VEGFB) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vascular Endothelial Growth Factor B (VEGFB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Vascular Endothelial Growth Factor B (VEGFB), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling | Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory | Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties | E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test | ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target | Vascular Endothelial Growth Factor B (VEGFB) |
| Short name | Vascular Endothelial Growth Factor B (VEGFB) ELISA kit |
| Technique | &hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host | antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name | Vascular Endothelial Growth Factor B (vascular endothelial growth factor B) Enzyme-linked immunosorbent assay reagent |
| Alternative technique | elisas |
| Alternative to gene target | Extracellular, VEGFB and IDBG-53284 and ENSG00000173511 and 7423, VEGFB and IDBG-643900 and ENSBTAG00000047567 and, VEGFL and VRF, Vegfb and IDBG-137732 and ENSMUSG00000024962 and 22340, protein heterodimerization activity, this GO :0001525 and angiogenesis and biological process this GO :0001938 and positive regulation of endothelial cell proliferation and biological process this GO :0002576 and platelet degranulation and biological process this GO :0005515 and protein binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005615 and extracellular space and cellular component this GO :0005622 and intracellular and cellular component this GO :0006493 and protein O-linked glycosylation and biological process this GO :0007507 and heart development and biological process this GO :0007596 and blood coagulation and biological process this GO :0008083 and growth factor activity and molecular function this GO :0008201 and heparin binding and molecular function this GO :0010629 and negative regulation of gene expression and biological process this GO :0016020 and membrane and cellular component this GO :0030168 and platelet activation and biological process this GO :0030949 and positive regulation of vascular endothelial growth factor receptor signaling pathway and biological process this GO :0031093 and platelet alpha granule lumen and cellular component this GO :0035470 and positive regulation of vascular wound healing and biological process this GO :0042056 and chemoattractant activity and molecular function this GO :0042493 and response to drug and biological process this GO :0042803 and protein homodimerization activity and molecular function this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0043183 and vascular endothelial growth factor receptor 1 binding and molecular function this GO :0043524 and negative regulation of neuron apoptotic process and biological process this GO :0046982 and protein heterodimerization activity and molecular function this GO :0048010 and vascular endothelial growth factor receptor signaling pathway and biological process this GO :0050731 and positive regulation of peptidyl-tyrosine phosphorylation and biological process this GO :0050918 and positive chemotaxis and biological process this GO :0050930 and induction of positive chemotaxis and biological process this GO :0051781 and positive regulation of cell division and biological process this GO :0051897 and positive regulation of protein kinase B signaling and biological process this GO :0060048 and cardiac muscle contraction and biological process this GO :0060754 and positive regulation of mast cell chemotaxis and biological process this GO :0060976 and coronary vasculature development and biological process this GO :0070374 and positive regulation of ERK1 and ERK2 cascade and biological process, this GO :0005515: protein binding, this GO :0005515: protein binding and also this GO :0008083: growth factor activity and also this GO :0008201: heparin binding and also this GO :0042056: chemoattractant activity and also this GO :0042803: protein homodimerization activity and also this GO :0043183: vascular endothelial growth factor receptor 1 binding and also this GO :0046982: protein heterodimerization activity, this GO :0008083: growth factor activity, this GO :0008201: heparin binding, this GO :0042056: chemoattractant activity, this GO :0042803: protein homodimerization activity, this GO :0043183: vascular endothelial growth factor receptor 1 binding, this GO :0046982: protein heterodimerization activity, vascular endothelial growth factor B |
| Identity | 12681 |
| Gene | VEGFB |
| Long gene name | vascular endothelial growth factor B |
| Synonyms gene | VRF |
| Synonyms | VEGFL |
| Locus | 11q13, 1 |
| Discovery year | 1996-10-26 |
| GenBank acession | BC008818 |
| Entrez gene record | 7423 |
| Pubmed identfication | 8637916 8919691 |
| RefSeq identity | NM_003377 |
| Classification | VEGF family |
| Havana BLAST/BLAT | OTTHUMG00000167813 |