| Long name | 114) Monoclonal Antibody, 163, VCP (1344CT150 |
| Also known as | 114) Monoclonal Antibody, 163, VCP (1344CT150 |
| Category | Primary Antibodies |
| Conjugation | Unconjugated |
| Target Antigen | VCP |
| Specificity | This is a highly specific antibody against VCP |
| Modification(s) | None |
| Modification site(s) | Unmodified antibody |
| Clonality | Monoclonal |
| Clone number | 114, 1344CT150, 163 |
| Source | This VCP antibody is generated from a mouse immunized with a recombinant protein from human VCP |
| Gene ID number | 7415 |
| Swiss Prot | P55072 |
| Tested Applications | IF, WB |
| Recommended dilutions | WB(1:1000), WB(1:1000) |
| Cross reactivity | Mouse, Rat, Human |
| Cross reactive species details | not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the target antigen | Also involved in DNA damage response: recruited to double-strand breaks (DSBs) sites in a RNF8- and RNF168-dependent manner and promotes the recruitment of TP53BP1 at DNA damage sites, Component of the VCP/p97-AMFR/gp78 complex that participates in the final step of the sterol-mediated ubiquitination and endoplasmic reticulum-associated degradation (ERAD) of HMGCR, Involved in the formation of the transitional endoplasmic reticulum (tER), Recruited to stalled replication forks by SPRTN: may act by mediating extraction of DNA polymerase eta (POLH) to prevent excessive translesion DNA synthesis and limit the incidence of mutations induced by DNA damage, Regulates E3 ubiquitin-protein ligase activity of RNF19A, Required for cytoplasmic retrotranslocation of stressed/damaged mitochondrial outer-membrane proteins and their subsequent proteasomal degradation, The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope, The ternary complex containing UFD1L, The transfer of membranes from the endoplasmic reticulum to the Golgi apparatus occurs via 50-70 nm transition vesicles which derive from part-rough, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, Vesicle budding from the tER is an ATP-dependent process, part-smooth transitional elements of the endoplasmic reticulum (tER), where they are degraded by the proteasome, Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis |
| Purification method | This antibody is purified through a protein G column, followed by dialysis against PBS, 09% (W/V) sodium azide, Purified monoclonal antibody supplied in PBS with 0 |
| Storage conditions | 50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to 1 year, Keep the antibody in aqueous buffered solution containing 1% BSA |
| Synonym names | 15S Mg(2+)-ATPase p97 subunit, ALS14, CMT2Y, HEL-220, HEL-S-70, IBMPFD, IBMPFD1, TER ATPase, TERA, Transitional endoplasmic reticulum ATPase, VCP, Valosin-containing protein, p97 |
| Properties | C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24° |
| About | Each murine monoclonal anibody has his own affinity specific for the clone, Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes, Monoclonals of this antigen are available in different clones |
| Gene target | 114) Monoclonal, 163, VCP (1344CT150 |
| Short name | 114) Monoclonal Antibody, 163, VCP (1344CT150 |
| Technique | Monoclonals or monoclonal antibodies, antibodies against human proteins, antibodies for, Antibody |
| Alternative name | 114) monoclonal (Antibody to), 163, valosin containing protein (1344CT150 |
| Alternative technique | antibodies |
| Alternative to gene target | ALS14 and HEL-220 and HEL-S-70 and IBMPFD and IBMPFD1 and p97 and TERA, BT, ER to cytosol and biological process this GO :0031334 and positive regulation of protein complex assembly and biological process this GO :0031593 and polyubiquitin binding and molecular function this GO :0032403 and protein complex binding and molecular function this GO :0032436 and positive regulation of proteasomal ubiquitin-dependent protein catabolic process and biological process this GO :0034214 and protein hexamerization and biological process this GO :0035800 and deubiquitinase activator activity and molecular function this GO :0035861 and site of double-strand break and cellular component this GO :0042802 and identical protein binding and molecular function this GO :0042981 and regulation of apoptotic process and biological process this GO :0043161 and proteasome-mediated ubiquitin-dependent protein catabolic process and biological process this GO :0043231 and intracellular membrane-bounded organelle and cellular component this GO :0043234 and protein complex and cellular component this GO :0043531 and ADP binding and molecular function this GO :0044822 and poly(A) RNA binding and molecular function this GO :0045184 and establishment of protein localization and biological process this GO :0045732 and positive regulation of protein catabolic process and biological process this GO :0048471 and perinuclear region of cytoplasm and cellular component this GO :0051260 and protein homooli this GO merization and biological process this GO :0070062 and extracellular vesicular exosome and cellular component this GO :0070842 and aggresome assembly and biological process this GO :1903006 and positive regulation of protein K63-linked deubiquitination and biological process this GO :1903007 and positive regulation of Lys63-specific deubiquitinase activity and biological process this GO :1990381 and ubiquitin-specific protease binding and molecular function, VCP and IDBG-61415 and ENSG00000165280 and 7415, Vcp and IDBG-139755 and ENSMUSG00000028452 and 269523, nuclei, this GO :0000502 and proteasome complex and cellular component this GO :0000836 and Hrd1p ubiquitin ligase complex and cellular component this GO :0005102 and receptor binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005783 and endoplasmic reticulum and cellular component this GO :0005789 and endoplasmic reticulum membrane and cellular component this GO :0005811 and lipid particle and cellular component this GO :0005829 and cytosol and cellular component this GO :0006200 and ATP catabolic process and biological process this GO :0006281 and DNA repair and biological process this GO :0006302 and double-strand break repair and biological process this GO :0006310 and DNA recombination and biological process this GO :0006511 and ubiquitin-dependent protein catabolic process and biological process this GO :0006888 and ER to this GO lgi vesicle-mediated transport and biological process this GO :0006919 and activation of cysteine-type endopeptidase activity involved in apoptotic process and biological process this GO :0006974 and cellular response to DNA damage stimulus and biological process this GO :0008289 and lipid binding and molecular function this GO :0009378 and four-way junction helicase activity and molecular function this GO :0016567 and protein ubiquitination and biological process this GO :0016787 and hydrolase activity and molecular function this GO :0016887 and ATPase activity and molecular function this GO :0018279 and protein N-linked glycosylation via asparagine and biological process this GO :0019903 and protein phosphatase binding and molecular function this GO :0019904 and protein domain specific binding and molecular function this GO :0019985 and translesion synthesis and biological process this GO :0030433 and ER-associated ubiquitin-dependent protein catabolic process and biological process this GO :0030968 and endoplasmic reticulum unfolded protein response and biological process this GO :0030970 and retrograde protein transport, this GO :0005102: receptor binding, this GO :0005102: receptor binding and also this GO :0005515: protein binding and also this GO :0005524: ATP binding and also this GO :0008289: lipid binding and also this GO :0009378: four-way junction helicase activity and also this GO :0016787: hydrolase activity and also this GO :0016887: ATPase activity and also this GO :0019903: protein phosphatase binding and also this GO :0019904: protein domain specific binding and also this GO :0031593: polyubiquitin binding and also this GO :0032403: protein complex binding and also this GO :0035800: deubiquitinase activator activity and also this GO :0042802: identical protein binding and also this GO :0043531: ADP binding and also this GO :0044822: poly(A) RNA binding and also this GO :1990381: ubiquitin-specific protease binding, this GO :0005515: protein binding, this GO :0005524: ATP binding, this GO :0008289: lipid binding, this GO :0009378: four-way junction helicase activity, this GO :0016787: hydrolase activity, this GO :0016887: ATPase activity, this GO :0019903: protein phosphatase binding, this GO :0019904: protein domain specific binding, this GO :0031593: polyubiquitin binding, this GO :0032403: protein complex binding, this GO :0035800: deubiquitinase activator activity, this GO :0042802: identical protein binding, this GO :0043531: ADP binding, this GO :0044822: poly(A) RNA binding, this GO :1990381: ubiquitin-specific protease binding, ubiquitin-specific protease binding, 49331 and IDBG-632847 and ENSBTAG00000015000 and 507345, valosin containing protein |
| Identity | 12666 |
| Gene | VCP |
| Long gene name | valosin containing protein |
| Synonyms gene name | valosin-containing protein |
| Synonyms | IBMPFD p97 CDC48 |
| Locus | 9p13, 3 |
| Discovery year | 1996-08-22 |
| GenBank acession | AC004472 |
| Entrez gene record | 7415 |
| Pubmed identfication | 8595912 7553851 |
| RefSeq identity | NM_007126 |
| Classification | AAA ATPases |
| Havana BLAST/BLAT | OTTHUMG00000019855 |
| Locus Specific Databases | LRG_657 |
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