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VGLL2 Antibody, FITC Conjugated

VGLL2 Antibody, FITC Conjugated size: 0.1ml 333

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 333
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name FITC Conjugated, VGLL2 Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenVGLL2
SpecificityThis is a highly specific antibody against VGLL2
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic peptide derived from human VGLL2
Gene ID number245806
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background During muscle differentiation, Overexpression of Vgl-2 in MYOD-transfected 10T1/2 mouse embryonic fibroblasts increases expression of myosin heavy chain (MHC), Specific to skeletal muscle, There are two isoforms of Vgl-2 that are produced as a result of alternative splicing events, This evidence suggests that Vgl-2 is essential for muscle gene expression, Vgl-2 is expressed highly in adult fast muscle and is expressed at lower levels in adult slow muscle and fetal skeletal muscle, Vgl-2 mRNA levels increase and Vgl-2 translocates from the cytoplasm to the nucleus, a protein that plays a role in controlling the expression of numerous genes, also known as VITO-1, is a 317 amino acid protein that contains a domain through which it interacts with TEF-1, which is a marker of terminal muscle differentiation, Vgl-2
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetVGLL2 Conjugated
Short name FITC Conjugated, VGLL2 Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, VGLL2 (Antibody to)
Alternative techniqueantibodies
Identity 20232
Gene VGLL2
Long gene name vestigial like family member 2
Synonyms gene name vestigial like 2 (Drosophila) vestigial-like family member 2
Locus 6q22, 1
Discovery year 2003-04-30
GenBank acession AY056583
Entrez gene record 245806
Pubmed identfication 12376544
RefSeq identity NM_153453
Classification Vestigial like family
Havana BLAST/BLAT OTTHUMG00000015451

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