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VGLL4 Antibody, Biotin Conjugated

VGLL4 Antibody, Biotin Conjugated size: 0.1ml 333

Price 333
Size 0.1ml
Long name Biotin Conjugated, VGLL4 Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationBiotinylated
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenVGLL4
SpecificityThis is a highly specific antibody against VGLL4
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic peptide derived from human VGLL4
Gene ID number9686
Tested applications IHC-P, WB
Recommended dilutions IHC-P(1:100-500), WB(1:100-1000)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Overexpression of Vgl-4 in cardiac myocytes interferes with basal expression and ?-adrenergic receptor-dependent activation of a TEF-1 dependent skeletal ?actin promoter, The mechanism whereby TEF-1 confers tissue specificity depends largely on the interaction of TEF-1 with tissue-specific cofactors, There are two isoforms of Vgl-4 that are produced as a result of alternative splicing events, This suggests that Vgl-4 counteracts ?-adrenergic activation of gene expression in cardiomyocytes, Transcription cofactor Vgl-4 (vestigial-like protein 4) is a 290 amino acid nuclear protein that interacts with TEF-1 and MEF-2, Vgl-4 is the only member of the vestigial-like family that is expressed in heart, The transcriptional enhancer factor-1 (TEF-1) family of transcription factors regulate tissue-specific gene expression in muscle and placenta
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation EmissionN/A
Synonyms KIAA0121
Properties Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetVGLL4 Conjugated
Short name Conjugated, VGLL4 Antibody
Technique antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelBiotin
Alternative name biotinilated coupled, VGLL4 (Antibody to)
Alternative techniqueantibodies
Identity 28966
Gene VGLL4
Long gene name vestigial like family member 4
Synonyms gene name vestigial like 4 (Drosophila) vestigial-like family member 4
Locus 3p25, 2 , 3-p25
Discovery year 2004-06-03
GenBank acession D50911
Entrez gene record 9686
Pubmed identfication 8590280 15140898
RefSeq identity NM_014667
Classification Vestigial like family
Havana BLAST/BLAT OTTHUMG00000129739

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