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Vimentin Antibody, FITC Conjugated

Vimentin Antibody, FITC Conjugated size: 0.1ml 333

Price 333
Size 0.1ml
Long name FITC Conjugated, Vimentin Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenVimentin
SpecificityThis is a highly specific antibody against Vimentin
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen_range416-466/466
Subcellular locationCytoplasm
SourceKLH conjugated synthetic peptide derived from human Vimentin
Gene ID number7431
Swiss ProtP08670
Tested applications IF(ICC), IF(IHC-P), FCM
Recommended dilutions IF(ICC)(1:50-200), IF(IHC-P)(1:50-200), FCM(1:20-100)
Crossreactivity Mouse, Pig, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2, Vimentin is attached to the nucleus, and mitochondria, either laterally or terminally, endoplasmic reticulum, especially mesenchymal cells, Vimentins are class-III intermediate filaments found in various non-epithelial cells
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Synonyms CTRCT3, VIM, Vimentin, HEL113
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetVimentin Conjugated
Short name FITC Conjugated, Vimentin Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, Vimentin (Antibody to)
Alternative techniqueantibodies

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