Filters

WISP3 Antibody, Biotin Conjugated

WISP3 Antibody, Biotin Conjugated size: 0.1ml 333

Price 333
Size 0.1ml
Long name Biotin Conjugated, WISP3 Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationBiotinylated
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenWISP3
SpecificityThis is a highly specific antibody against WISP3
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic peptide derived from human WISP3
Gene ID number8838
Tested applications IHC-P, WB
Recommended dilutions IHC-P(1:100-500), WB(1:100-1000)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Both WISP-1 and WISP-2 are upregulated in cells transformed with the proto-oncogene Wnt-1, WISP-1, WISP-1 expression is observed in various tissues including adult heart, WISP-2 and WISP-3 are members of the CCN family of growth factors, WISP-2 and WISP-3 share significant sequence similarity, WISP-3 is involved in normal post-natal skeletal growth, and it is also implicated in the development of the autosomal recessive skeletal disorder progressive pseudorheumatoid dysplasia, and they are also more highly expressed in human colon tumors, and they are believed to function as dimers in their active forms, colon and ovary, including four conserved cysteine-rich domains, kidney and spleen, suggesting that these proteins may participate in tumor development, which affects cartilage homeostasis by disrupting the growth of chondrocyte and normal cell columnar organization, which include connective tissue growth factor (CTGF) and Cyr61, while WISP-2 expression predominates in skeletal muscle, Wnt-induced secreted protein (WISP)-1
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation EmissionN/A
Synonyms CCN6
Properties Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetWISP3 Conjugated
Short name Conjugated, WISP3 Antibody
Technique antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelBiotin
Alternative name biotinilated coupled, WISP3 (Antibody to)
Alternative techniqueantibodies
Identity 12771
Gene WISP3
Long gene name WNT1 inducible signaling pathway protein 3
Locus 6q21
Discovery year 1999-01-22
GenBank acession AF100781
Entrez gene record 8838
Pubmed identfication 9843955
RefSeq identity NM_003880
Classification CYR61/CTGF/NOV matricellular proteins
Havana BLAST/BLAT OTTHUMG00000185101

Subscribe to our Newsletter