Name : WISP3 Antibody, Biotin Conjugated
Supplier : BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price :333
SKU : GEN4397240149
| Long name | Biotin Conjugated, WISP3 Polyclonal Antibody |
| Category | Conjugated Primary Antibodies |
| Conjugation | Biotinylated |
| Host Organism | Rabbit (Oryctolagus cuniculus) |
| Target Antigen | WISP3 |
| Specificity | This is a highly specific antibody against WISP3 |
| Modification | Unmodified |
| Modification site | None |
| Clonality | Polyclonal |
| Clone | Polyclonal antibody |
| Concentration | 1ug per 1ul |
| Source | KLH conjugated synthetic peptide derived from human WISP3 |
| Gene ID number | 8838 |
| Tested applications | IHC-P, WB |
| Recommended dilutions | IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity | Mouse, Rat, Human |
| Crossreactive species details | not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background | Both WISP-1 and WISP-2 are upregulated in cells transformed with the proto-oncogene Wnt-1, WISP-1, WISP-1 expression is observed in various tissues including adult heart, WISP-2 and WISP-3 are members of the CCN family of growth factors, WISP-2 and WISP-3 share significant sequence similarity, WISP-3 is involved in normal post-natal skeletal growth, and it is also implicated in the development of the autosomal recessive skeletal disorder progressive pseudorheumatoid dysplasia, and they are also more highly expressed in human colon tumors, and they are believed to function as dimers in their active forms, colon and ovary, including four conserved cysteine-rich domains, kidney and spleen, suggesting that these proteins may participate in tumor development, which affects cartilage homeostasis by disrupting the growth of chondrocyte and normal cell columnar organization, which include connective tissue growth factor (CTGF) and Cyr61, while WISP-2 expression predominates in skeletal muscle, Wnt-induced secreted protein (WISP)-1 |
| Purification method | This antibody was purified via Protein A |
| Storage conditions | 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission | N/A |
| Synonyms | CCN6 |
| Properties | Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target | WISP3 Conjugated |
| Short name | Conjugated, WISP3 Antibody |
| Technique | antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label | Biotin |
| Alternative name | biotinilated coupled, WISP3 (Antibody to) |
| Alternative technique | antibodies |
| Identity | 12771 |
| Gene | WISP3 |
| Long gene name | WNT1 inducible signaling pathway protein 3 |
| Locus | 6q21 |
| Discovery year | 1999-01-22 |
| GenBank acession | AF100781 |
| Entrez gene record | 8838 |
| Pubmed identfication | 9843955 |
| RefSeq identity | NM_003880 |
| Classification | CYR61/CTGF/NOV matricellular proteins |
| Havana BLAST/BLAT | OTTHUMG00000185101 |