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ZAP70 (49B4) Monoclonal Antibody

ZAP70 (49B4) Monoclonal Antibody size: 0.1ml 362

Supplier BIOSS PRIMARY UNCONJUGATED ANTIBODIES
Price 362
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long nameZAP70 (49B4) Monoclonal Antibody
Also known asZAP70 (49B4) Monoclonal Antibody
CategoryPrimary Antibodies
ConjugationUnconjugated
Target AntigenZAP70
SpecificityThis is a highly specific antibody against ZAP70
Modification(s)None
Modification site(s)Unmodified antibody
ClonalityMonoclonal
Clone number49B4
Concentration1ug per 1ul
SourceRecombinant human protein purified from E, coli (ABD-ZAP70)
Gene ID number7535
Swiss ProtP43403
Tested Applications IP, WB
Recommended dilutions0ug), 1ug/mL) IP (2, WB (0
Cross reactivityHuman
Cross reactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Background of the target antigen Additionally, Contributes also to the development and activation of primary B-lymphocytes, During thymocyte development, Furthermore, In turn, Regulates motility, Release of ZAP70 active conformation is further stabilized by phosphorylation mediated by LCK, Subsequently, T-cell proliferation and differentiation, This recruitment serves to localization to the stimulated TCR and to relieve its autoinhibited conformation, Thus, When antigen presenting cells (APC) activate T-cell receptor (TCR), ZAP70 controls cytoskeleton modifications, ZAP70 is also required for TCR-CD247/CD3Z internalization and degradation through interaction with the E3 ubiquitin-protein ligase CBL and adapter proteins SLA and SLA2, ZAP70 phosphorylates at least 2 essential adapter proteins: LAT and LCP2, ZAP70 promotes survival and cell-cycle progression of developing thymocytes before positive selection (when cells are still CD4/CD8 double negative), ZAP70 regulates both T-cell activation switch on and switch off by modulating TCR expression at the T-cell surface, ZAP70-dependent signaling pathway may also contribute to primary B-cells formation and activation through B-cell receptor (BCR), a large number of signaling molecules are recruited and ultimately lead to lymphokine production, a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD247/CD3Z through ITAM motif at the plasma membrane, adhesion and cytokine expression of mature T-cells, adhesion and mobility of T-lymphocytes, as well as thymocyte development, thus ensuring correct delivery of effectors to the APC, Tyrosine kinase that plays an essential role in regulation of the adaptive immune response
Purification methodPurified by Protein G
Storage conditions 50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to 1 year, Keep the antibody in aqueous buffered solution containing 1% BSA
Synonym names 70 kDa zeta-chain associated protein, ADMIO2, IMD48, STCD, STD, Syk-related tyrosine kinase, TZK, Tyrosine-protein kinase ZAP-70, ZAP-70, ZAP70, SRK
PropertiesC, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°
About Each murine monoclonal anibody has his own affinity specific for the clone, Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes, Monoclonals of this antigen are available in different clones
Gene targetZAP70 (49B4) Monoclonal
Short nameZAP70 (49B4) Monoclonal Antibody
Technique Monoclonals or monoclonal antibodies, antibodies against human proteins, antibodies for, Antibody
Alternative namezeta-chain (TCR) associated protein kinase 70kDa (49B4) monoclonal (Antibody to)
Alternative techniqueantibodies
Alternative to gene target Cell surfaces, SRK and STCD and STD and TZK and ZAP-70, ZAP70 and IDBG-62509 and ENSG00000115085 and 7535, ZAP70 and IDBG-629842 and ENSBTAG00000005892 and 504509, Zap70 and IDBG-145089 and ENSMUSG00000026117 and 22637, this GO :0001772 and immunological synapse and cellular component this GO :0001784 and phosphotyrosine binding and molecular function this GO :0002250 and adaptive immune response and biological process this GO :0004672 and protein kinase activity and molecular function this GO :0004713 and protein tyrosine kinase activity and molecular function this GO :0004715 and non-membrane spanning protein tyrosine kinase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005911 and cell-cell junction and cellular component this GO :0006468 and protein phosphorylation and biological process this GO :0006955 and immune response and biological process this GO :0016772 and transferase activity, this GO :0001784: phosphotyrosine binding, this GO :0001784: phosphotyrosine binding and also this GO :0004672: protein kinase activity and also this GO :0004713: protein tyrosine kinase activity and also this GO :0004715: non-membrane spanning protein tyrosine kinase activity and also this GO :0005515: protein binding and also this GO :0005524: ATP binding and also this GO :0016772: transferase activity, this GO :0004672: protein kinase activity, this GO :0004713: protein tyrosine kinase activity, this GO :0004715: non-membrane spanning protein tyrosine kinase activity, this GO :0005515: protein binding, this GO :0005524: ATP binding, this GO :0016772: transferase activity, transferase activity, transferring phosphorus-containing groups, transferring phosphorus-containing groups, transferring phosphorus-containing groups and molecular function this GO :0018108 and peptidyl-tyrosine phosphorylation and biological process this GO :0030217 and T cell differentiation and biological process this GO :0035556 and intracellular signal transduction and biological process this GO :0042101 and T cell receptor complex and cellular component this GO :0042110 and T cell activation and biological process this GO :0042113 and B cell activation and biological process this GO :0043366 and beta selection and biological process this GO :0045059 and positive thymic T cell selection and biological process this GO :0045060 and negative thymic T cell selection and biological process this GO :0045061 and thymic T cell selection and biological process this GO :0045121 and membrane raft and cellular component this GO :0045582 and positive regulation of T cell differentiation and biological process this GO :0046638 and positive regulation of alpha-beta T cell differentiation and biological process this GO :0046641 and positive regulation of alpha-beta T cell proliferation and biological process this GO :0046777 and protein autophosphorylation and biological process this GO :0050850 and positive regulation of calcium-mediated signaling and biological process this GO :0050852 and T cell receptor signaling pathway and biological process this GO :0070489 and T cell aggregation and biological process this GO :0072678 and T cell migration and biological process, zeta-chain (TCR) associated protein kinase 70kDa
Identity 12858
Gene ZAP70
Long gene name zeta chain of T-cell receptor associated protein kinase 70
Synonyms gene SRK
Synonyms gene name zeta-chain (TCR) associated protein kinase (70 kD) zeta-chain (TCR) associated protein kinase 70kDa zeta chain of T cell receptor associated protein kinase 70kDa zeta chain of T cell receptor associated protein kinase 70
Synonyms ZAP-70 STD
Synonyms name tyrosine-protein kinase ZAP-70
Locus 2q11, 2
Discovery year 1995-05-16
GenBank acession L05148
Entrez gene record 7535
Pubmed identfication 1423621
RefSeq identity NM_001079
Classification SH2 domain containing
Havana BLAST/BLAT OTTHUMG00000153060
Locus Specific Databases ZAP70base: Mutation registry for autosomal recessive ZAP70 immunodeficiency LRG_126

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